PMID: 6161970Jan 1, 1980Paper

Immunoperoxidase procedures to detect monoclonal antibodies against cell surface antigens. Quantitation of binding and staining of individual cells

Journal of Immunological Methods
P M LansdorpW P Zeijlemaker

Abstract

An immunoperoxidase method has been developed which allows accurate and sensitive quantitation of the binding of monoclonal antibodies to cell surface antigens. Monolayers of fixed cells were prepared in wells of Terasaki micro-test plates and monoclonal antibodies bound to cell surface antigens were identified by the unlabeled antibody-enzyme method of Sternberger (1974). The cell-bound peroxidase could either be quantified per well or visualized on individual cells by the use of appropriate substrates for peroxidase. Experimental procedures are described in detail and results obtained with several monoclonal antibodies with specificity for different target cells are shown. Limitations and applications of the technique are discussed.

References

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Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·M NovákováP Dr-aber
Aug 12, 1983·Journal of Immunological Methods·L De LeijT H The
Sep 16, 1983·Journal of Immunological Methods·V Horejsí, I Hilgert
Jun 10, 1983·Journal of Immunological Methods·B Holzmann, J P Johnson
Aug 2, 1985·Journal of Immunological Methods·B S ThomsenG Bendixen
Feb 11, 1985·Journal of Immunological Methods·P CoppeR Leloup
May 1, 1986·Journal of Immunological Methods·M Lefroit-Joliy, T Neveu
Feb 26, 1987·Journal of Immunological Methods·H Durbin, W F Bodmer
Aug 3, 1987·Journal of Immunological Methods·M H Bocchieri
Feb 11, 1987·Journal of Immunological Methods·H BaumgartenO Götze
Mar 16, 1988·Journal of Immunological Methods·H OkadaS Ferrone
May 8, 1990·Journal of Immunological Methods·K CarrollR O'Kennedy
Apr 8, 1991·Journal of Immunological Methods·M C Cerrone, R E Kuhn
Apr 12, 1995·Journal of Immunological Methods·P DráberM Nováková
Dec 1, 1983·Molecular Immunology·W L BigbeeR H Jensen
Jan 1, 1987·Acta histochemica·P DráberV Viklický
Apr 1, 1996·Acta histochemica·C J VissersF T Bosman
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J HeyesJ Trowsdale
Aug 10, 2000·Journal of Immunoassay·J E Butler
May 1, 1993·The Journal of Experimental Medicine·W CraigP M Lansdorp
Nov 1, 1983·British Journal of Haematology·P A TetterooA E von dem Borne
Sep 1, 1984·Journal of Clinical Pathology·C A MakinW F Bodmer
Feb 12, 2008·Blood·Peter M Lansdorp
Sep 1, 1994·Journal of Neurosurgery·S TanakaS Yasumoto

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