PMID: 8943743Oct 1, 1996Paper

Immunoperoxidase staining for identification of Aspergillus species in routinely processed tissue sections

Journal of Clinical Pathology
P E VerweijJ F Meis

Abstract

To evaluate the performance of an immunoperoxidase stain using the monoclonal antibody EB-A1 to detect Aspergillus species in formalin fixed, paraffin wax embedded tissue. The monoclonal antibody EB-A1 directed against galactomannan was used to detect Aspergillus species in 23 patients with suspected or confirmed invasive aspergillosis. Immunostaining was performed on formalin fixed, paraffin wax embedded tissue using the streptavidin-biotin method and compared with conventional haematoxylin and eosin, periodic acid-Schiff, and Gomori-Grocott stains. Results of immunostaining were semiquantitatively analysed with regard to both intensity of staining and number of positively staining micro-organisms. Tissue sections from 16 patients with confirmed invasive mycoses due to Candida species, Apophysomyces elegans, Rhizopus oryzae, Pseudallescheria boydii and Histoplasma capsulatum were used as controls. In 19 (83%) of 23 cases invasive aspergillosis was confirmed by both histological examination and culture (18 Aspergillus fumigatus and one A flavus). Immunoperoxidase stains were positive in 17 (89%) of 19 cases including one case of disseminated infection due to A flavus. Furthermore, the immunoperoxidase stain was positive in a cu...Continue Reading

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Citations

Sep 6, 2001·Diagnostic Cytopathology·A E Walts
Sep 29, 2011·Current Infectious Disease Reports·Cornelia Mrazek, Cornelia Lass-Flörl
May 9, 2012·Folia Microbiologica·Lenka Bašková, Vladimír Buchta
Nov 17, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rocus R KlontPaul E Verweij
Dec 3, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P J PatersonC C Kibbler
Jul 16, 2009·Applied Immunohistochemistry & Molecular Morphology : AIMM·Audrey N Schuetz, Cynthia Cohen
Jan 31, 2004·American Journal of Clinical Pathology·John K ChoiKarin L McGowan
Aug 23, 2008·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Yoshinari MyokenYoshinori Fujita
Feb 12, 2009·Mycoses·Suganthini KrishnanPranatharthi H Chandrasekar
Apr 11, 2008·Mycoses·P K ShuklaG B S Keshava
Sep 27, 2005·The Lancet Infectious Diseases·W W HopeD W Denning
Aug 5, 2017·Applied Immunohistochemistry & Molecular Morphology : AIMM·Martina PejchalDouglas J Hartman
Mar 6, 2008·Archives of Pathology & Laboratory Medicine·Eduardo Eyzaguirre, Abida K Haque

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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