PMID: 6168556Jul 1, 1981Paper

Immunoperoxidase staining in the recognition of Aspergillus infections

Histopathology
E N Saeed, R J Hay

Abstract

This study concerns the use of the indirect immunoperoxidase technique for labelling Aspergillus species in infected human and animal tissues in smears taken from cultures. Specific labelling without serious cross-reactivity with Candida species or Zygomycete fungi was demonstrated using antiserum to Aspergillus. However, it was not possible to distinguish between two Aspergillus species. A fumigatus and A. flavus with this technique. Pretreatment of sections with sodium azide and normal swine serum was effective in eliminating background staining. The method can be used to produce permanently stained sections and has applications in the recognition of hyphal fragments in tissue from infected patients.

References

Oct 1, 1979·Journal of Clinical Pathology·E Heyderman
May 1, 1977·Annals of Internal Medicine·J AisnerS C Schimpff
Oct 1, 1969·American Journal of Clinical Pathology·W Kaplan, D E Kraft
Nov 1, 1973·The American Journal of Tropical Medicine and Hygiene·B VeressA M el-Hassan
May 1, 1967·The Journal of Cell Biology·P K Nakane, G B Pierce
Feb 1, 1981·Journal of Medical Microbiology·V M Hearn, D W Mackenzie

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Citations

Jan 1, 1982·Transactions of the Royal Society of Tropical Medicine and Hygiene·R J Hay, D W Mackenzie
Jul 16, 2009·Applied Immunohistochemistry & Molecular Morphology : AIMM·Audrey N Schuetz, Cynthia Cohen
Apr 1, 1996·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H E JensenL Kaufman
Jun 1, 2017·Frontiers in Microbiology·Guillaume Desoubeaux, Carolyn Cray

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