PMID: 6164880Oct 1, 1980Paper

Immunoperoxidase staining of cerebrospinal fluid IgG in isoelectric focusing gels: a sensitive new technique

Journal of Neuroscience Methods
D H MattsonB G Arnason

Abstract

An isoelectric focusing (IEF) technique using direct peroxidase or peroxidase-antiperoxidase (PAP) overlay staining for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a specific stain. The direct peroxidase system is 4 times and the PAP system 8 times as sensitive as Coomassie blue staining after standard IEF or Coomassie blue staining of immunofixed gels. As little as 0.5 microgram (direct peroxidase) or 0.3 microgram (PAP) of IgG can be detected. Direct peroxidase staining of IgG in IEF gels can be used to examine unconcentrated CSF, with banding patterns similar to those obtained by Coomassie blue staining after standard IEF of concentrated CSF.

References

Apr 1, 1979·Journal of Neurology, Neurosurgery, and Psychiatry·M A Laurenzi, H Link
Oct 16, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·S N Kahn, E J Thompson
Nov 15, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·C H Chang, N R Inglis
Oct 1, 1974·Journal of the Neurological Sciences·K G Kjellin, O Vesterberg

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Citations

Feb 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·F GauthierH Mouray
Aug 1, 1983·Journal of Neuroscience Methods·R P Roos, M Lichter
May 1, 1993·Journal of Neuroimmunology·G MartinoL M Grimaldi
Dec 1, 1982·Journal of Clinical Pathology·M H Johnson, E J Thompson
Jun 1, 1982·Journal of Neuroimmunology·D H MattsonB G Arnason
Mar 1, 1982·In Practice·I Griffiths
Feb 1, 1988·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·B H BassG P Rice
Jan 1, 1985·Neurochemical Pathology·K W Rammohan
Feb 1, 1986·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·K G WarrenD J Carroll

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