Immunophenotyping and characterization of BNP colostra revealed pathogenic alloantibodies of IgG1 subclass with specifity to platelets, granulocytes and monocytes of all maturation stages

Veterinary Immunology and Immunopathology
Aryan AssadCornelia A Deeg

Abstract

Bovine neonatal pancytopenia (BNP) is mainly characterized by multiple haemorrhages, thrombocytopenia and leukocytopenia as a result of bone marrow depletion. BNP can be induced in healthy calves through application of colostrum from BNP donors, proofing that BNP is mediated to maternal alloantibodies. Alloantibody binding to bovine blood cells is present in sera and colostra of BNP donors and is probably initialized by vaccination with a certain BVD vaccine. To understand etiology and pathomechanisms of BNP, we closely characterized disease inducing antibodies regarding immunoglobulin subclass and binding specificities to peripheral blood derived leukocytes and platelets. By exact phenotyping the targeted blood cell subsets, including platelets for the first time, we investigated that BNP alloantibodies are exclusively of IgG1 subclass. Interestingly, IgG1 of BNP colostra bound to 70% leukocytes and 100% platelets irrespective of different bovine breeds and cellular maturity of all specimens tested. Furthermore, staining pattern on platelets as well as leukocyte subsets by BNP-IgG1 alloantibody exposed 100% reactivity to platelets, granulocytes and monocytes. Interestingly, the main part of T-helper cells was not bound by colo...Continue Reading

References

Oct 31, 1996·Nature·A K AbbasA Sher
Feb 15, 2001·Journal of Hematotherapy & Stem Cell Research·S Agrawal, M C Kishore
Aug 3, 2001·Vaccine·P MoingeonA Lindberg
Jun 18, 2002·Blood Cells, Molecules & Diseases·M Tarek Elghetany
Jun 20, 2002·Veterinary Immunology and Immunopathology·L B Corbeil
Sep 27, 2005·The Journal of Dairy Research·Balázs MayerImre Kacskovics
Jan 15, 2008·Veterinary Immunology and Immunopathology·Ratree PlattJames A Roth
Feb 19, 2009·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Hagop BessosStanislaw J Urbaniak
Feb 27, 2009·Veterinary Immunology and Immunopathology·Kuldeep S ChatthaPatricia E Shewen
Dec 29, 2009·Nature Medicine·Andreas GreinacherJürgen Bux
Jun 22, 2010·Transfusion·Angelika ReilJuergen Bux
Oct 15, 2010·Blood·Beth H ShazChristopher D Hillyer
Oct 16, 2010·Nature Reviews. Immunology·Michael R Ehrenstein, Clare A Notley
Jan 26, 2011·The Veterinary Record·C R BellC D Penny
Jan 29, 2011·Veterinary Immunology and Immunopathology·Philip Simon BridgerKlaus Doll
Feb 22, 2011·BMC Veterinary Research·Annette FriedrichCarola M Sauter-Louis
Sep 21, 2011·Veterinary Immunology and Immunopathology·Armando HeriazonBonnie A Mallard

❮ Previous
Next ❯

Citations

Nov 2, 2016·Expert Review of Vaccines·Lindert Benedictus, Charlotte R Bell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.