Immunophenotyping of chronic B-cell neoplasms: flow cytometry versus immunohistochemistry.

Hematology Reports
Afaf Abdel-Aziz Abdel-GhafarYasmin Nabil El-Sakhawy

Abstract

Morphological differentiation between benign and malignant lymphoproliferative disorders (LPDs) can be challenging. Immunophenotyping (IPT) by either technique, flow cytometry or immunohistochemistry (IHC), is an important step in solving such difficulty. Thirty-five newly diagnosed patients with chronic B-cell neoplasms (11 chronic lymphocytic leukemia, 22 non Hodgkin lymphoma and 2 hairy cell leukemia) were included in this study with age range from 20 to 70 years. Monoclonal antibodies surface expression using lymphoproliferative disorders panel (CD45, CD19, CD5, CD10, CD11c, CD20, CD22, CD23, CD38, CD79b, FMC7, CD103, CD25, kappa and lambda light chains) by flow cytometry was done on bone marrow samples. CD20, CD5, CD23, Bcl-2, Bcl-6, kappa and lambda light chain immunostaining were performed on fixed bone marrow trephine biopsy specimen. The sensitivity of IHC was 81.8% in chronic lymphocytic leukemia (CLL) and 100% in non Hodgkin lymphoma (NHL) as regards CD20, 100% in both groups as regards CD5, 46% in CLL and 66.7% in NHL as regards CD23, 33.3% in CLL and 50% in NHL as regards kappa chain, 20% in CLL and 33.3% in NHL as regards lambda chain. We found that IHC and flow cytometry are equally effective in diagnosing CLL; h...Continue Reading

Citations

Sep 18, 2012·Biochimica Et Biophysica Acta·Paul Faustin Seke EtetArmel Hervé Nwabo Kamdje
Sep 29, 2020·Expert Review of Clinical Pharmacology·J M BurkeJ P Sharman
Jul 8, 2021·Journal of the Royal Society, Interface·Petros X E MouratidisGail Ter Haar
Aug 21, 2021·Forensic Science, Medicine, and Pathology·Lorenzo GittoSerenella Serinelli

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Methods Mentioned

BETA
flow
biopsy
light microscopy
flow cytometry
biopsies

Software Mentioned

Colomo
SPSS

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