PMID: 9191883Jul 1, 1997Paper

Immunophenotyping of T lymphocytes by three-color flow cytometry in healthy newborns, children, and adults

Clinical Immunology and Immunopathology
T W McCloskeyS Pahwa

Abstract

Reagents are now available which allow simultaneous assessment of three different fluorescence wave-lengths on most commercially available flow cytometers. Such three-color analyses provide more information than single- or dual-color analyses. The present study was undertaken in order to establish age-related differences in lymphocyte subpopulations by simultaneously measuring three surface antigens in newborns, children, and adults. A whole blood method was used to label cells with antibodies conjugated to FITC, PE, and perCP. We found that the percentage of lymphocytes expressing HLA-DR/CD28/CD8, HLA-DR/ CD38/CD8, CD95/CD45RO/CD8, CD95/CD45RO/CD4, CD95/CD4, and CD95/CD8 showed relative increases with age. The percentage of lymphocytes expressing CD28/CD8, CD38/CD8, and CD38/CD4 showed relative decreases with age, while the subset HLA-DR/CD38/ CD4 did not change. Three-color flow cytometry is a powerful tool to more precisely define lymphocyte subsets than the current two-color methods. We present values using a three-color panel in healthy newborns, children, and adults.

References

Aug 1, 1991·Clinical Immunology and Immunopathology·T ReichertJ Linden
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R B CorleyG Haughton
Nov 1, 1986·Pediatric Research·Y YanaseT Kawasaki
Dec 1, 1986·The Nurse Practitioner·S Loken
Jan 1, 1984·Cancer Investigation·F Traganos
Jun 1, 1982·The Journal of Cell Biology·V T OiL Stryer
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E L ReinherzS F Schlossman
Jan 1, 1980·The Journal of Experimental Medicine·D T YuH G Kunkel
Apr 1, 1994·Clinical Immunology and Immunopathology·S Plaeger-MarshallE R Stiehm
Sep 1, 1994·Journal of Clinical Immunology·M C AldhousK S Froebel
Nov 1, 1994·Scandinavian Journal of Immunology·A CarusoA Balsari
Apr 1, 1994·Clinical Immunology and Immunopathology·C JenningsA Landay

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Citations

Nov 12, 2003·The Journal of Allergy and Clinical Immunology·William T ShearerUNKNOWN Pediatric AIDS Clinical Trials Group
Oct 24, 2000·Journal of Immunological Methods·T J StabelT E Rahner
Dec 2, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·L C GrammerC L Goolsby
Jan 1, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jutarat MekmullicaKenneth McIntosh
Jul 17, 1998·The Journal of Clinical Investigation·F D GoldmanN Taylor
Dec 15, 2005·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Jacob KarshRobert E Dales
Apr 15, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Víctor Sánchez-MargaletRaimundo Goberna
May 6, 2008·Clinics in Perinatology·Dolly Sharma, Paul Spearman
Jun 14, 2005·European Journal of Clinical Investigation·P P SfikakisM A Dimopoulos
Jun 6, 2003·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Helena A Zdolsek, Maria C Jenmalm
Jan 13, 2000·American Journal of Clinical Pathology·M I FielH C Bodenheimer
Apr 5, 2005·The Journal of Allergy and Clinical Immunology·Mary E PaulUNKNOWN Women and Infants Transmission Study
Apr 3, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·E OnoM I de Moraes-Pinto
Nov 20, 2002·American Journal of Obstetrics and Gynecology·Helene B BernsteinAudrey L Kinter
Nov 27, 2014·Memórias do Instituto Oswaldo Cruz·Maria Isabel de Moraes-PintoReinaldo Salomão
Nov 9, 2002·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D A O QueirózK E Stewien
Oct 17, 2006·Cytometry. Part B, Clinical Cytometry·Carlos Alberto Zaccarelli-FilhoMaria Isabel de Moraes-Pinto
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A SavarinoU Dianzani
Dec 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jojanneke HeidemaGrada M van Bleek
Jul 8, 2000·American Journal of Clinical Pathology·S D Hudnall, C P Molina
Feb 12, 2021·Frontiers in Immunology·Jamal Hussen, Hans-Joachim Schuberth
Aug 10, 2001·The Journal of Allergy and Clinical Immunology·M E PaulD E Lewis

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