Immunopotentiation of Polysaccharides of Atractylodes macrocephala Koidz-loaded nanostructured lipid carriers as an adjuvant

International Journal of Biological Macromolecules
Zhenguang LiuDeyun Wang

Abstract

The immunoregulation and immunopotentiation of Polysaccharides of Atractylodes macrocephala Koidz (PAMK) have been widely demonstrated. Nanostructured lipid carriers (NLC) have high drug loading capacity for lipophilic and hydrophilic drugs, and have good biocompatibility and high bioavailability. In this study, the effect of PAKM-NLC on the surface molecule expression of bone marrow-derived dendritic cells (BMDCs) in vitro was investigated by flow cytometry, and the cytokines secreted by dendritic cell supernatants were detected by ELISA. The results showed that compared with other control groups, PAMK-NLC could significantly increase the expression of CD80 and CD86 and promote the secretion of IL-1β, IL-12, TNF-α and IFN-γ, indicating that PAMK-NLC have a more pronounced effect on the maturation and differentiation of BMDCs. In addition, effects of PAMK-NLC nanoparticles on OVA-immunized mice were explored. Compared with other control groups, PAMK-NLC-OVA can significantly promote the production of OVA-specific antibodies in serum, stimulate the secretion of cytokines, increase the proliferation rate of spleen lymphocytes after OVA re-stimulation, and induce stronger activation of CD3+CD4+ and CD3+CD8+ lymphocytes. As an adju...Continue Reading

References

Jan 13, 2001·Current Opinion in Immunology·C Théry, S Amigorena
Dec 10, 2002·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Raymond Chang
May 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tazio Storni, Martin F Bachmann
Jun 4, 2005·Advances in Experimental Medicine and Biology·Chandrashekhar Pasare, Ruslan Medzhitov
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Sue D XiangMagdalena Plebanski
Jul 3, 2007·Advanced Drug Delivery Reviews·R H MüllerJ Pardeike
Sep 18, 2007·Nature Biotechnology·Sai T ReddyJeffrey A Hubbell
Jul 1, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Julia M Martínez GómezPål Johansen
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hélène Jusforgues-SaklaniMatthew L Albert
Dec 19, 2012·International Journal of Biological Macromolecules·Zhenjie ZhangFengping Shan
May 27, 2014·International Immunopharmacology·Jong-Jin KimSung-Tae Yee
Jun 1, 2014·Comparative Immunology, Microbiology and Infectious Diseases·Chetan V Jawale, John Hwa Lee
Jan 29, 2016·International Journal of Biological Macromolecules·Bing HanJin Pei
Feb 27, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hui WangShun-Qi Wang
Mar 7, 2018·Journal of the American Pharmacists Association : JAPhA·Youqi Zhang, Mary S Hayney

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Citations

Sep 26, 2020·PLoS Neglected Tropical Diseases·Xuemin JinMingyuan Liu
Oct 30, 2020·International Journal of Biological Macromolecules·Yu ZhengXiaofang Li
Aug 20, 2021·International Journal of Biological Macromolecules·Xiuge GaoDawei Guo

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