Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody.

Methods in Molecular Biology
Hunter R Gibbons, Thomas M Aune

Abstract

Formation of DNA:RNA hybrids or R-loops contributes to numerous biologic processes. The development of the S9.6 antibody makes the analysis of R-Loops (DNA:RNA hybrids) possible through immunoprecipitation. Here, we describe the isolation of DNA:RNA hybrid structures using the S9.6 antibody. Using this protocol, both the DNA and RNA binding partners of the R-loop can be analyzed via qPCR, whole genome sequencing, or other methods.

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