Immunoprecipitation of surface antigen precursors from Schistosoma mansoni messenger RNA in vitro translation products

Molecular and Biochemical Parasitology
D W TaylorA E Butterworth

Abstract

Messenger RNA has been isolated from adults and eggs of Schistosoma mansoni and translated in vitro in mRNA dependent rabbit reticulocyte lysate. The in vitro translation products have been immunoprecipitated using a wide variety of hyperimmune and infection sera from rabbits, mice and humans. A large number of in vitro translation products are precipitated by these sera, and we have identified a subset of the immunoprecipitated polypeptides which are expressed on the surface of the young schistosomulum. Messenger RNA from both adults and eggs directs the synthesis of these polypeptides suggesting that at least some of the surface proteins of the young schistosomulum are being synthesised throughout the life cycle. cDNA clone banks prepared from adult mRNA will therefore contain the genes for these schistosomulum surface antigens greatly facilitating their isolation.

Citations

Feb 15, 1991·Experientia·D W Taylor
Apr 15, 1986·Experientia·P Köhler
Apr 1, 1992·International Journal for Parasitology·A M Tenter, A M Johnson
Jan 1, 1985·Transactions of the Royal Society of Tropical Medicine and Hygiene·A E ButterworthJ H Ouma
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·A J SimpsonS R Smithers
Sep 1, 1985·Molecular and Biochemical Parasitology·D P McManusA J Simpson
Sep 1, 1985·Molecular and Biochemical Parasitology·D A HarnJ R David
May 1, 1985·Molecular and Biochemical Parasitology·R J PierceA Capron
Apr 1, 1986·Molecular and Biochemical Parasitology·D O Irving, M J Howell
Mar 1, 1986·Molecular and Biochemical Parasitology·D W TaylorJ E McMahon
Dec 1, 1987·Molecular and Biochemical Parasitology·J A UpcroftP Upcroft
Sep 1, 1988·Molecular and Biochemical Parasitology·H Profous-JuchelkaM Turner
Jul 1, 1988·Molecular and Biochemical Parasitology·J C HavercroftA E Butterworth
Aug 1, 1989·Molecular and Biochemical Parasitology·K S JohnsonJ S Cordingley
Feb 1, 1990·Molecular and Biochemical Parasitology·K P IamsA J Musoke
Apr 1, 1991·Molecular and Biochemical Parasitology·P O AliA J Simpson
Jul 1, 1993·Molecular and Biochemical Parasitology·F G AbathA J Simpson
Nov 12, 1996·Molecular and Biochemical Parasitology·P R GardinerM W Clarke
Jun 1, 1984·Veterinary Parasitology·D W TaylorA E Butterworth
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M K AbbasG D Cain
Jun 1, 1987·Diagnostic Microbiology and Infectious Disease·S E Maddison
Oct 1, 1987·Parasitology·C KellyS R Smithers
Oct 1, 1988·Parasitology·M LastreA Capron
Jan 1, 1986·Parasitology·D W TaylorA E Butterworth
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A H DavisA Mahmoud
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Xu, R V Lewis
May 1, 1991·Parasite Immunology·A N Schweitzer, D W Taylor
Mar 12, 1987·Nature·F E Cox
Mar 12, 1987·Nature·J M BalloulA Capron
Jan 1, 1994·Archives of Orthopaedic and Trauma Surgery·N Y Otsuka, J Schatzker

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