Immunoprecipitation

Current Protocols in Protein Science
J S BonifacinoT A Springer

Abstract

Immunoprecipitation is a technique in which an antigen is isolated by binding to a specific antibody attached to a sedimentable matrix. It is also used to analyze protein fractions separated by other biochemical techniques such as gel filtration or density gradient sedimentation. The source of antigen for immunoprecipitation can be unlabeled cells or tissues, metabolically or intrinsically labeled cells, or in-vitro-translated proteins. This unit describes a wide range of immunoprecipitation techniques, using either suspension or adherent cells lysed by various means (e.g., with and without detergent, using glass beads, etc.). Flow charts and figures give the user a clear-cut explanation of the options for employing the technology.

References

Jan 1, 1978·Journal of Immunological Methods·D M Gersten, J J Marchalonis
Jan 1, 1979·Methods in Enzymology·A HeleniusC Tanford
Jan 1, 1991·Methods in Enzymology·A FranzusoffR Schekman
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Dec 22, 1970·Biochimica Et Biophysica Acta·J A van Mourik, I A Mochtar
Jan 1, 1984·Methods in Enzymology·L M Hjelmeland, A Chrambach
Jan 1, 1983·Methods in Enzymology·D J Anderson, G Blobel
Apr 1, 1995·Molecular Biotechnology·R Rapley

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Citations

Oct 5, 2016·Scientific Reports·Jarosław KróliczewskiBożena Króliczewska
Dec 24, 2010·The Plant Journal : for Cell and Molecular Biology·Olga BlifernezOlaf Kruse
Jun 23, 2009·FEBS Letters·Jonelle L Mattiacio, Laurie K Read
May 28, 2021·Nano Letters·Youcef Chakib HaceneNicolas Bertrand
Feb 3, 2006·Genes & Development·Yang David Lee, Stephen J Elledge

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