Immunoproteasome-specific inhibitors and their application

Methods in Molecular Biology
Michael Basler, M Groettrup

Abstract

Immunoproteasomes (IPs) containing the interferon-inducible subunits β1i (LMP2), β2i (MECL-1), and β5i (LMP7) alter proteasomal cleavage preference, optimise the generation of peptide ligands of MHC class I molecules, alter cytokine profile, influence T-helper cell differentiation, and play a role in T-cell survival. Small molecule inhibitors are useful tools for probing the role of the immunoproteasome in immune functions. Here, we describe different methods to characterise immunoproteasome-selective inhibitors. Thereby, we provide the methodology to analyse the specificity and cell permeability of immunoproteasome inhibitors, as well as to functionally investigate immunoproteasome inhibitors in antigen presentation.

Citations

Nov 19, 2014·Immunogenetics·Sonja Erath, Marcus Groettrup
Jun 27, 2019·Pharmacology Research & Perspectives·Katrien PletinckxTorsten R Dunkern
Oct 17, 2017·British Journal of Pharmacology·Michael BaslerMarcus Groettrup
Jul 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael BaslerMarcus Groettrup
Jul 19, 2012·Chembiochem : a European Journal of Chemical Biology·Lalit Kumar SharmaKyung-Bo Kim
Oct 27, 2017·European Journal of Immunology·Michael BaslerMarcus Groettrup
Aug 26, 2020·Genes and Immunity·Michael Basler, Marcus Groettrup
Oct 30, 2020·Drug Design, Development and Therapy·Yu CaoJiankang Zhang
Jul 9, 2021·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Ying FangJinhai Wang

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