Immunoproteomic identification of 11 novel immunoreactive proteins of Riemerella anatipestifer serotype 2

FEMS Immunology and Medical Microbiology
Zhipeng ZhaiWei Zhang

Abstract

Riemerella anatipestifer (RA) is one of the most important bacterial pathogens of ducks and other avian species worldwide. Twenty-one serotypes of RA have been identified, with RA serotype two (RA2) being reported as one of the most predominant serotypes underlying infections in China. Current approaches to the control of RA are hindered by the absence of effective vaccines, particularly those that exhibit cross-protection between different serotypes. In this study, a combination of two-dimensional electrophoresis, Western blot analysis and mass spectrometry were used to identify the antigenic proteins of RA2. A total of 16 immunoreactive proteins, representing 12 distinct proteins, were identified. These included OmpA, a known immunogenic protein of RA, as well as novel immunogens. PCR analysis also indicated that genes corresponding to each of the 12 distinct proteins were conserved among different RA serotypes. Eleven genes encoding these proteins were cloned and expressed in Escherichia coli BL21 (DE3). Eight of the 11 expressed proteins were able to react with hyperimmune rabbit serum against RAf153. One of these, recombinant elongation factor G, responded to RA2 sera but not RA1, whereas recombinant OmpA responded to both...Continue Reading

References

Sep 1, 1989·The British Veterinary Journal·L M Timms, R N Marshall
Apr 16, 1985·Biochemical and Biophysical Research Communications·J P Aris, R D Simoni
Oct 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D J SpeareN MacNair
Apr 1, 1997·International Journal of Systematic Bacteriology·S SubramaniamJ Frey
Jan 1, 1997·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·S DheinW Klaus
Feb 11, 1998·Journal of Bacteriology·R A GarduñoP S Hoffman
Mar 7, 2000·Clinical and Diagnostic Laboratory Immunology·S SubramaniamJ Frey
Mar 16, 2000·Developmental and Comparative Immunology·D A HigginsZ V Kounev
Jul 11, 2000·Microbiology and Immunology·H HaraK Fukui
Jan 25, 2003·Diseases of Aquatic Organisms·Jari MadetojaTom Wiklund
Feb 13, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Chao-Fu ChangYung-Fu Chang
Jan 18, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jennifer L HessMichael D P Boyle
Mar 15, 2005·Avian Pathology : Journal of the W.V.P.A·Hsiang-Jung TsaiMing-Jeng Pan
Jun 28, 2005·Molecular Microbiology·Jesper E Mogensen, Daniel E Otzen
Oct 27, 2006·Clinical and Vaccine Immunology : CVI·R R KulkarniJ F Prescott
Feb 10, 2007·Autoimmunity Reviews·Benjamin TerrierLuc Mouthon
Nov 6, 2007·Analytical Biochemistry·Jaana MuhonenSusanne K Wiedmer
Feb 21, 2008·Current Opinion in Chemical Biology·Hsing-Ju WuMichael P Jennings
May 17, 2008·Avian Pathology : Journal of the W.V.P.A·A TurbahnE F Kaleta
Dec 1, 1996·Avian Pathology : Journal of the W.V.P.A·P PathanasophonT Tanticharoenyos
Jul 8, 2009·Veterinary Parasitology·Liheng LiuXiangrui Li
Nov 3, 2010·The Veterinary Journal·Paulina K BeltránAngel H Alvarez
Dec 25, 2010·Journal of Bacteriology·Zutao ZhouMing Sun
Feb 26, 2011·Veterinary Microbiology·Qinghai HuShengqing Yu
Mar 29, 2011·Journal of Bacteriology·Jianfeng YuanSongnian Hu

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