Immunoquantitative analysis of artemisinin from Artemisia annua using polyclonal antibodies.

Phytochemistry
J F Ferreira, J Janick

Abstract

Artemisinin was derivatized to dihydroartemisinin carboxymethylether in three steps, without disturbing the peroxide bridge, and then linked to either thyroglobulin (TGB) or bovine serum albumin (BSA). The artemisinin-TGB and -BSA conjugates were injected in female New Zealand rabbits but only the artemisinin-TGB conjugate generated polyclonal antibodies. An enzyme-linked immunosorbent assay (ELISA) was developed and the specificity of the antibodies was confirmed by comparison with pre-immune serum and by competitive assays using different dilutions of artemisinin standards. Although anti-artemisinin antibodies cross-reacted with artemisitene and dihydroartemisinin at all dilutions used, cross-reaction with deoxyartemisinin, artemisinic acid, and arteannuin B occurred only at high concentrations. ELISA successfully detected artemisinin from crude extracts in concentrations as low as 1.5 ng ml-1; and was epsilon 400-fold more sensitive than the HPLC-EC. The ELISA successfully detected and quantified artemisinin in different organs of greenhouse-grown plants and in eight clones of Artemisia annua grown in tissue culture but artemisinin was overestimated owing to cross-reactivity of the antibodies with artemisinin-related compoun...Continue Reading

References

Oct 1, 1985·Planta medica·N ActonI J Rollman

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Citations

Jan 6, 1999·Archives of Pharmacal Research·D W Jung, C K Sung
Sep 8, 2006·Analytical and Bioanalytical Chemistry·Nan LiuBoyang Yu
Jun 15, 2006·Applied Microbiology and Biotechnology·Chunzhao LiuYuchun Wang
Feb 9, 2000·Life Sciences·V DhingraM Lakshmi Narasu
Sep 21, 2000·Plant Science : an International Journal of Experimental Plant Biology· Van Geldre E Van den Eeckhout E
Feb 24, 2010·Indian Journal of Pharmaceutical Sciences·S P AgarwalShipra Ahuja
Apr 18, 2014·Drug Development and Industrial Pharmacy·Richard A GravesLevon A Bostanian
Jan 17, 2008·Phytochemical Analysis : PCA·Paula C CastilhoAna I Rodrigues
Aug 19, 2015·Analytical and Bioanalytical Chemistry·Laura ZehnackerStéphane Azoulay
Oct 2, 2013·The American Journal of Tropical Medicine and Hygiene·Min WangBaomin Wang
Jan 28, 2017·Monatshefte für chemie·Armin PresserSilke Buzzi

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