PMID: 107506Jan 1, 1979

Immunoradiometric and electroimmuno assay of increased ferritin and apoferritin levels in serum

Pathology
R D BrownH Kronenberg

Abstract

Ferritin in serum from patients with increased serum ferritin levels has been studied both quantitatively and qualitatively. All techniques utilized in these studies are suitable to be used as routine screening tests for large numbers of patients. Electroimmuno assay (EIA) has been compared with the solid phase immunoradiometric (IRMA) assay as a technique to determine serum ferritin concentration (r = 0.99) and is suggested as a useful alternative when determining ferritin concentrations above 500 microgram/l. Iron stained EIA gels have been used to indicate the iron content of the ferritin molecule in sera. This simple screening test has demonstrated that apoferritin is found more often than iron-rich ferritin in the serum of patients with elevated serum ferritin levels. Immunoelectrophoresis precipitin bands suggest the heterogeneity of ferritin in serum from different patients.

References

Mar 8, 1975·British Medical Journal·S HusseinJ F Moorhead
May 1, 1975·The New England Journal of Medicine·A Jacobs, M Worwood
Apr 12, 1975·British Medical Journal·D P Bentley, A Jacobs
Feb 8, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·J W HallidayL W Powell
May 1, 1975·Clinical Science and Molecular Medicine·M WorwoodA Jacobs
Aug 1, 1974·Journal of Clinical Pathology·E A LetskyD M Flynn
Oct 1, 1974·Journal of Clinical Pathology·D P Bentley, P Williams
Sep 1, 1974·Analytical Biochemistry·L E MilesJ D Cook
Sep 1, 1974·British Journal of Haematology·M WorwoodJ A Whittaker
Jun 24, 1971·The New England Journal of Medicine·R R Crichton
Sep 1, 1971·Journal of Clinical Pathology·M R BeamishA Jacobs
Jun 1, 1956·The Journal of Clinical Investigation·K R REISSMANN, M R DIETRICH

Citations

Jan 1, 1981·Journal of Biological Standardization·C McLeod, W Katz
Oct 1, 1984·Australian and New Zealand Journal of Medicine·R J TrentH Kronenberg
Dec 1, 1979·British Journal of Haematology·G CaseyR J Kimber
Jan 1, 1983·Pathology·R D BrownH Kronenberg
Feb 1, 1988·European Journal of Haematology·R D BrownH Kronenberg

Related Concepts

Ferritin Light Chain
Electrochemistry
Isoferritin
Immunodiffusion Measurement
Liver
Radioimmunoassay

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.