PMID: 7390878Jan 1, 1980Paper

Immunoreactivities of gastrin (G-) cells. II. Non-specific binding of immunoglobulins to G-cells by ionic interactions

Histochemistry
D Grube

Abstract

Various gastro-entero-pancreatic (GEP) endocrine cells have been shown to contain concomitantly immunoreactivities against several peptide hormones. In the present study the "immunoreactivities" of gastrin (G-) cells of the rat stomach against 21 specific antisera and 10 control sera were investigated by means of the unlabelled antibody enzyme (PAP) technique using modifications of single steps in the immunocytochemical staining sequence. The results indicate that immunoglobulins can bind to gastrin cell granules obviously by non-specific ionic interactions. This non-specific binding of immunoglobulins occurs even in dilution ranges of the sera commonly used in immunohistochemical investigations of the GEP endocrine system. Since "adsorption controls" (preadsorption of the antisera with their respective antigens) will not discriminate between specific and non-specific binding of immunoglobulins to GEP endocrine cells additional specificity controls are necessary. In contrast to the immunostaining of various GEP endocrine cells by "established" antisera and of G-cells by gastrin antiserum immunoglobulins of sera from non-immunized animals as well as antibodies against corticotropin-lipotropin related peptides could be displaced ...Continue Reading

References

Aug 12, 1976·Histochemistry·B Ghiringehello, G Bussolati
Jun 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J W BigbeeL F Eng
Feb 4, 1978·Lancet·P J Lowry, L H Rees
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B E NoyesK L Agarwal
Dec 13, 1978·Histochemistry·J AlumetsF Sundler
May 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D F SwaabF W Van Leeuwen
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E WeberR Martin
Dec 28, 1978·Histochemistry·D GrubeE Weber
Oct 1, 1979·The Journal of Clinical Endocrinology and Metabolism·J F BruniS S Yen
Apr 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G ItohI Suzuki
Aug 1, 1977·Life Sciences·R B LuftigP N McMillan
May 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L I LarssonE Solcia
Nov 9, 1978·Cell and Tissue Research·N S TrackW Creutzfeldt
Oct 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P PetruszP DiMeo
Oct 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L I Larsson, T W Schwartz
Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·R E Coalson
Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·W G Forssmann, L Orci
Apr 1, 1961·The Journal of Biophysical and Biochemical Cytology·H D MAYORB ROSARIO
Nov 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H MAYERSBACH

❮ Previous
Next ❯

Citations

Jan 1, 1985·Histochemistry·F C Guinel, M E McCully
Jan 1, 1989·The Histochemical Journal·U SchumacherE Kaiserling
Mar 1, 1983·The Histochemical Journal·F T Bosman
Feb 1, 1993·Current Eye Research·G E MarshallW R Lee
Feb 21, 2002·The Journal of Veterinary Medical Science·Raden Harry SoehartonoHaruo Yamada
Jan 1, 2010·Archives of Histology and Cytology·Maiko Haga, Sumio Yoshie
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Peter Petrusz
Mar 1, 1988·The Anatomical Record·R CalvertJ Ferrari
Jan 1, 1983·Acta Neuropathologica·J UlrichA Probst
Nov 1, 1982·Experimental Lung Research·L H LazarusC M Soldato
Jan 1, 1984·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·P FritzG Wegner
Jul 25, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·W KodaY Nakanuma
Oct 8, 2004·Archives of Histology and Cytology·Dietrich Grube
Apr 1, 1990·Cell and Tissue Research·C VaillantR M Batt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.