PMID: 2510809Jan 1, 1989Paper

Immunoreactivity of hormonally-characterized human endocrine cells against three novel anti-human chromogranin B(B11 and B13) and chromogranin A (A11) monoclonal antibodies

Archives of Histology and Cytology
R BuffaE Solcia

Abstract

Two novel monoclonal antibodies, called B11 and B13, directed exclusively against human chromogranin B (CgB) and another antibody, A11, specific for human chromogranin A (CgA), were obtained by immunization of mice with chromaffin granules, the fusion of their splenocytes, the screening of hybridomas supernatants by ELISA and immunohistochemistry, and characterization of the antibodies by two-dimensional immunoblotting. The antibodies were used in immunohistochemical tests to investigate the distribution of CgA and CgB in hormonally-identified cells of the human endocrine system. The A11 antibody confirmed the occurrence of CgA in gut EC, ECL, gastrin, secretin and neurotensin cells, pancreatic A and PP cells, parathyroid chief cells, pituitary TSH and gonadotrope cells and adrenal medullary cells. Only a fraction of CgA-immunoreactive cells in the human gut and pancreas showed C-terminus arginine-glycinamide immunoreactivity, suggesting pancreastatin storage. Both CgB antibodies showed immunoreactivity in gastrin cells, intestinal (but not gastric) EC cells, pancreatic A and PP cells, pituitary TSH and gonadotrope cells and adrenal medullary cells. In addition, the B11 antibody stained thyroid C cells and the B13 antibody stai...Continue Reading

Citations

Aug 1, 1992·Neuroscience·H Winkler, R Fischer-Colbrie
Mar 10, 2000·Regulatory Peptides·E KarlssonS Sandler
Apr 25, 2012·World Journal of Gastroenterology : WJG·Sébastien A B RoyNathalie Perreault
Jun 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G M Portela-GomesL Grimelius
Jul 23, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Guida Maria Portela-Gomes, Mats Stridsberg
Dec 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Songyan WangBurton M Wice
May 1, 1993·The Anatomical Record·D Adriaensen, D W Scheuermann
Jan 1, 1991·Acta Oncologica·W E Schmidt, W Creutzfeldt

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