Abstract
Pluripotent stem cell markers can be useful for diagnostic evaluation of human tumors. The novel pluripotent marker stage-specific embryonic antigen-5 (SSEA-5) is expressed in undifferentiated human induced pluripotent cells (iPSCs), but little is known about SSEA-5 expression in other primitive tissues (e.g., human tumors). We evaluated SSEA-5 immunoreactivity patterns in human tumors, cell lines, teratomas, and iPS cells together with another pluripotent cell surface marker L1 cell adhesion molecule (L1CAM). We tested two hypotheses: (1) SSEA-5 and L1CAM would be immunoreactive and colocalized in human tumors; (2) SSEA-5 and L1CAM immunoreactivity would persist in iPSCs following retinal differentiating treatment. SSEA-5 immunofluorescence was most pronounced in primitive tumors, such as embryonal carcinoma. In tumor cell lines, SSEA-5 was highly immunoreactive in Capan-1 cells, while L1CAM was highly immunoreactive in U87MG cells. SSEA-5 and L1CAM showed colocalization in undifferentiated iPSCs, with immunopositive iPSCs remaining after 20 days of retinal differentiating treatment. This is the first demonstration of SSEA-5 immunoreactivity in human tumors and the first indication of SSEA-5 and L1CAM colocalization. SSEA-5 an...Continue Reading
References
Nov 7, 2006·Trends in Biotechnology·Hannes HentzeAlan Colman
Dec 25, 2007·Nature·In-Hyun ParkGeorge Q Daley
Aug 5, 2008·Cancer Research·Shideng BaoJeremy N Rich
Dec 17, 2008·Current Stem Cell Research & Therapy·Jan S Moreb
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jason S MeyerDavid M Gamm
Jan 12, 2011·Biochemical Pharmacology·Xin LiAi-Ming Yu
Feb 4, 2011·Nature·Ryan ListerJoseph R Ecker
Mar 4, 2011·Nature·Athurva GoreKun Zhang
Aug 16, 2011·Nature Biotechnology·Chad TangMicha Drukker
Oct 1, 2011·Stem Cells·Yeon Sung SonHyo Jeong Hong
Jun 22, 2012·Medical Molecular Morphology·Yoko MatsudaToshiyuki Ishiwata
Sep 25, 2012·Stem Cells and Development·John W RiggsPaul S Knoepfler
Oct 13, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Hee Jin LeeMyoung Sik Han
Nov 9, 2012·Neurology India·Kiran Krishne GowdaRakesh K Vasishta
Dec 21, 2012·Pathology International·Koji OkudelaKenichi Ohashi