Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells

Journal of Biomarkers
Linda CassidyGail M Seigel

Abstract

Pluripotent stem cell markers can be useful for diagnostic evaluation of human tumors. The novel pluripotent marker stage-specific embryonic antigen-5 (SSEA-5) is expressed in undifferentiated human induced pluripotent cells (iPSCs), but little is known about SSEA-5 expression in other primitive tissues (e.g., human tumors). We evaluated SSEA-5 immunoreactivity patterns in human tumors, cell lines, teratomas, and iPS cells together with another pluripotent cell surface marker L1 cell adhesion molecule (L1CAM). We tested two hypotheses: (1) SSEA-5 and L1CAM would be immunoreactive and colocalized in human tumors; (2) SSEA-5 and L1CAM immunoreactivity would persist in iPSCs following retinal differentiating treatment. SSEA-5 immunofluorescence was most pronounced in primitive tumors, such as embryonal carcinoma. In tumor cell lines, SSEA-5 was highly immunoreactive in Capan-1 cells, while L1CAM was highly immunoreactive in U87MG cells. SSEA-5 and L1CAM showed colocalization in undifferentiated iPSCs, with immunopositive iPSCs remaining after 20 days of retinal differentiating treatment. This is the first demonstration of SSEA-5 immunoreactivity in human tumors and the first indication of SSEA-5 and L1CAM colocalization. SSEA-5 an...Continue Reading

References

Nov 7, 2006·Trends in Biotechnology·Hannes HentzeAlan Colman
Aug 5, 2008·Cancer Research·Shideng BaoJeremy N Rich
Dec 17, 2008·Current Stem Cell Research & Therapy·Jan S Moreb
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jason S MeyerDavid M Gamm
Jun 22, 2012·Medical Molecular Morphology·Yoko MatsudaToshiyuki Ishiwata
Sep 25, 2012·Stem Cells and Development·John W RiggsPaul S Knoepfler
Oct 13, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Hee Jin LeeMyoung Sik Han

❮ Previous
Next ❯

Citations

Oct 15, 2019·Oncoscience·Gail M SeigelJidong Shan

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

Prism
Spot Advanced
SSEA

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
Feng YeHuaizeng Chen
Proceedings of the National Academy of Sciences of the United States of America
Jamie M SpergerJames A Thomson
Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology
T Motoyama
© 2021 Meta ULC. All rights reserved