PMID: 6172150Dec 21, 1981Paper

Immunoreactivity of subunits of the (Na+ + K+)-ATPase. Cross-reactivity of the alpha, alpha + and beta forms in different organs and species

Biochimica Et Biophysica Acta
G D SchellenbergW L Stahl

Abstract

The immunologic cross-reactivity of the alpha and alpha + forms of the large subunit and the beta subunit of the (Na+ + K+)-ATPase from brain and kidney preparations was examined using rabbit antiserum prepared against the purified holo lamb kidney enzyme. As previously reported by Sweadner ((1979) J. Biol. Chem. 254, 6060-6067) phosphorylation of the large subunit of the (Na+ + K+)-ATPase in the presence of Na+, Mg2+, and [gamma-32P]ATP revealed that dog and, very likely, rat brain contain two forms of the large subunit (designated alpha and alpha +) while dog, rat, and lamb kidney contain only one form (alpha). The cross-reactivity of the alpha and alpha + forms in these preparations was investigated by resolving the subunits by SDS-polyacrylamide gel electrophoresis. The separated polypeptides were transferred to unmodified nitrocellulose paper and reacted with rabbit anti-lamb kidney serum, followed by detection of the antigen-antibody complex with 125I-labeled protein A and autoradiography. By this method, the alpha and alpha + forms of rat and dog brain, as well as the alpha form found in kidney, were shown to cross-react. In addition, membranes from human cerebral cortex were shown to contain two immunoreactive bands cor...Continue Reading

References

Jun 18, 1971·Biochemical and Biophysical Research Communications·J Kyte
Jan 1, 1980·The International Journal of Biochemistry·E H Petrali, P V Sulakhe

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Citations

Jan 1, 1984·Annals of Neurology·W L Stahl
Jan 1, 1982·Histochemistry·D G Baskin, W L Stahl
Jul 1, 1988·The Journal of Membrane Biology·P L Jørgensen, J P Andersen
Jun 1, 1984·Journal of Bioenergetics and Biomembranes·W H PetersS L Bonting
Jun 1, 1984·Neurochemical Research·I V Pech, W L Stahl
Aug 1, 1990·The American Journal of Physiology·R Blostein, E Grafova
Mar 1, 1985·The American Journal of Physiology·A McDonough, C Schmitt
Jun 1, 1986·The American Journal of Physiology·R A FarleyA Kudrow
Sep 11, 1986·Biochimica Et Biophysica Acta·T Matsuda, H Iwata
Jul 11, 1985·Biochimica Et Biophysica Acta·T MatsudaJ R Cooper
Jan 29, 1990·Biochimica Et Biophysica Acta·I Berrebi-BertrandL G Lelièvre
May 9, 1989·Biochimica Et Biophysica Acta·K J Sweadner
Jan 1, 1986·Neurochemistry International·W L Stahl
May 1, 1985·Experimental Eye Research·A McDonough
Oct 1, 1987·Archives of Biochemistry and Biophysics·T Matsuda, H Iwata
Oct 30, 1985·Biochemical and Biophysical Research Communications·J Lytton

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