Immunoregulation of bovine macrophages by factors in the salivary glands of Rhipicephalus microplus.

Parasites & Vectors
Danett K Brake, Adalberto A Pérez de León

Abstract

Alternative strategies are required to control the southern cattle tick, Rhipicephalus microplus, due to evolving resistance to commercially available acaricides. This invasive ectoparasite is a vector of economically important diseases of cattle such as bovine babesiosis and anaplasmosis. An understanding of the biological intricacies underlying vector-host-pathogen interactions is required to innovate sustainable tick management strategies that can ultimately mitigate the impact of animal and zoonotic tick-borne diseases. Tick saliva contains molecules evolved to impair host innate and adaptive immune responses, which facilitates blood feeding and pathogen transmission. Antigen presenting cells are central to the development of robust T cell responses including Th1 and Th2 determination. In this study we examined changes in co-stimulatory molecule expression and cytokine response of bovine macrophages exposed to salivary gland extracts (SGE) obtained from 2-3 day fed, pathogen-free adult R. microplus. Peripheral blood-derived macrophages were treated for 1 hr with 1, 5, or 10 μg/mL of SGE followed by 1, 6, 24 hr of 1 μg/mL of lipopolysaccharide (LPS). Real-time PCR and cytokine ELISA were used to measure changes in co-stimula...Continue Reading

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Citations

Sep 24, 2013·Experimental & Applied Acarology·Munyaradzi C MarufuMichael Chimonyo
Sep 13, 2013·Parasites & Vectors·Nina M PooleJudith A Cole
Nov 19, 2013·Parasites & Vectors·Bruna BizzarroAnderson Sá-Nunes
Nov 15, 2012·Case Reports in Medicine·F Z Fdili AlaouiK Ait Taleb
Jun 24, 2014·Ticks and Tick-borne Diseases·Ntanganedzeni O MapholiKennedy Dzama
May 3, 2012·International Journal for Parasitology·Felix D GuerreroAdalberto A Pérez de León
Jul 21, 2015·Journal of Proteomics·Jan KotálJindřich Chmelař
Jan 15, 2015·Parasites & Vectors·Tamires Marielem Carvalho-CostaCarlo Jose Freire Oliveira
Mar 25, 2015·Ticks and Tick-borne Diseases·Verónica Díaz-MartínRicardo Pérez-Sánchez
Jan 27, 2015·Ticks and Tick-borne Diseases·Lorena Lopes FerreiraLígia Miranda Ferreira Borges
Oct 6, 2012·Chemico-biological Interactions·Kevin B TemeyerAdalberto A Pérez de León
Jun 18, 2017·Genetics, Selection, Evolution : GSE·Bruna P SolleroFernando F Cardoso

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Methods Mentioned

BETA
Protein Assay
electrophoresis
PCR
ELISA

Software Mentioned

Softmax Pro

Related Concepts

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Babesiosis (ASM)

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.