Immunoregulatory role of IL-35 in T cells of patients with rheumatoid arthritis

Rheumatology
Souichiro NakanoYoshinari Takasaki

Abstract

IL-35 is the most recently identified member of the IL-12 family. It consists of EBV-induced gene 3 (EBI3) and IL-12α chain p35. We investigated whether IL-35 enhances the in vitro immunosuppressive function of peripheral blood isolated from patients with RA. Peripheral blood was harvested from 17 active and 10 inactive RA patients and IL-35 concentrations were quantified using an ELISA. An expression vector containing IL-35 with a FLAG tag at the carboxyl-terminus was constructed by covalently linking EBI3 and IL-12α (p35). The function of IL-35 was then evaluated in a suppression assay using T cells isolated from human RA patients with CD2, CD3 and CD28 antibodies. Serum IL-35 levels and the number of Treg were decreased significantly in patients with active RA. There was a significant correlation between serum IL-35 and the 28-joint DAS with ESR (DAS28-ESR) in patients with active RA. IL-35 treatment enhanced the regulatory function, suppressing the levels of inflammatory cytokines such as IL-17 and IFN-γ and the cellular growth of effector T cells stimulated by conjugation with CD2, CD3 and CD28. These data revealed that IL-35 might suppress T cell activation during the peripheral immune responses of RA. Therefore our data ...Continue Reading

References

Jan 11, 2003·Science·Shohei HoriShimon Sakaguchi
Mar 4, 2003·Nature Immunology·Jason D FontenotAlexander Y Rudensky
Mar 4, 2003·Nature Immunology·Roli KhattriFred Ramsdell
Jul 29, 2004·The Journal of Experimental Medicine·Michael R EhrensteinClaudia Mauri
Mar 16, 2006·Blood·Xavier ValenciaPeter E Lipsky
Jan 4, 2007·The Journal of Experimental Medicine·Suchita NadkarniMichael R Ehrenstein
Jan 16, 2007·Nature·Marc A GavinAlexander Y Rudensky
Nov 23, 2007·Nature·Lauren W CollisonDario A A Vignali
Jan 24, 2009·Immunological Reviews·Lauren W Collison, Dario A A Vignali
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lauren W CollisonDario A A Vignali
Dec 8, 2009·Rheumatic Diseases Clinics of North America·Jaap Fransen, Piet L C M van Riel
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irina KochetkovaDavid W Pascual
Oct 19, 2010·Nature Immunology·Lauren W CollisonDario A A Vignali
May 18, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vandana ChaturvediDario A A Vignali
Feb 7, 2012·Nature Immunology·Lauren W CollisonDario A A Vignali

❮ Previous
Next ❯

Citations

Oct 21, 2015·Nature Reviews. Rheumatology·Louisa E JefferyMartin Hewison
Sep 16, 2015·The Tohoku Journal of Experimental Medicine·Xingwang NingWei Wang
Oct 4, 2016·Inflammation·Shi-Yang GuanHai-Feng Pan
Jun 24, 2017·International Immunopharmacology·Siqi LiuZhenjing Jin
Jul 29, 2018·Clinical and Experimental Medicine·Ya SuQijian Yi
Sep 27, 2018·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Meiling HanJing Lu
Feb 9, 2018·Nature Reviews. Disease Primers·Josef S SmolenKazuhiko Yamamoto
Jul 6, 2019·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Qinglai JiangJing Lu
Nov 16, 2018·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Müçteba Enes YaylaAşkin Ateş
Dec 14, 2018·Experimental and Therapeutic Medicine·Xia ZhangYing Liu
May 12, 2018·Immunologic Research·Lazaros I SakkasDimitrios P Bogdanos
Dec 12, 2019·Frontiers in Immunology·Benoit BrillandJean-François Augusto
Mar 22, 2020·International Journal of Molecular Sciences·Yosuke KamiyaAkio Mitani
Dec 12, 2020·International Immunopharmacology·Qiang XieAn-Fang Huang
Apr 19, 2021·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Li-Xin FuZai-Pei Guo
Jun 1, 2021·Frontiers in Pharmacology·Pan Lin XinCheng Wen Dan
Nov 18, 2021·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Cheng YeDario A A Vignali

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.