PMID: 18720820Aug 30, 2008Paper

Immunoresonance scattering spectral assay for the determination of antithrombin-III

Guang pu xue yu guang pu fen xi = Guang pu
Bo Jiang, Zhiliang Jiang

Abstract

A new sensitive, selective and simple immune resonance scattering spectral assay was proposed for the determination of trace amounts of Antithrombin-III (AT-III). It was based on the immune reaction of AT-III with the goat-anti-human AT-III antibody and the resonance scattering effect of the immunocomplex particles in pH 7.2 Tris-HCl buffer solution and in the presence of polyethelene glycol (PEG)6000. The results showed that the resonance scattering signal of AT-III and goat-anti-human AT-III antibody was very weak. However, AT-III was combined with goat-anti-human AT-III antibody specifically, and aggregated to form immunocomplex particles, which enhanced the resonance scattering intensity greatly and produced three resonance scattering peaks at 368 nm, 491 nm and 536 nm respectively. The strongest resonance scattering peak was at 491 nm. In the present paper, the influences of pH, goat-anti-human AT-III antibody and PEG-6000 concentration, incubation temperature, reactive time, and foreign substances were investigated. The result showed that the resonance scattering intensity at 491 nm (I(RS)) is linear to the AT-III concentration in the range of 62.5 to 850 ng x mL(-1), under the optimum conditions of 0.30 mL goat-anti-huma...Continue Reading

Related Concepts

Related Feeds

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

Related Papers

Guang pu xue yu guang pu fen xi = Guang pu
Wen-Sheng MaZhiliang Jiang
Guang pu xue yu guang pu fen xi = Guang pu
Jun-yao DengAihui Liang
Guang pu xue yu guang pu fen xi = Guang pu
Xiao-Ling WeiZhiliang Jiang
Guang pu xue yu guang pu fen xi = Guang pu
Aihui Liang, Sumei Wang
© 2022 Meta ULC. All rights reserved