Immunosensor for Pancreatic Cancer Based on Electrospun Nanofibers Coated with Carbon Nanotubes or Gold Nanoparticles

ACS Omega
Juliana C SoaresOsvaldo N Oliveira

Abstract

We report the fabrication of immunosensors based on nanostructured mats of electrospun nanofibers of polyamide 6 and poly(allylamine hydrochloride) coated either with multiwalled carbon nanotubes (MWCNTs) or gold nanoparticles (AuNPs), whose three-dimensional structure was suitable for the immobilization of anti-CA19-9 antibodies to detect the pancreatic cancer biomarker CA19-9. Using impedance spectroscopy, the sensing platform was able to detect CA19-9 with a detection limit of 1.84 and 1.57 U mL-1 for the nanostructured architectures containing MWCNTs and AuNPs, respectively. The high sensitivity achieved can be attributed to the irreversible adsorption between antibodies and antigens, as confirmed with polarization-modulated infrared reflection absorption spectroscopy. The adsorption mechanism was typical Langmuir-Freundlich processes. The high sensitivity and selectivity of the immunosensors were also explored in tests with blood serum from patients with distinct concentrations of CA19-9, for which the impedance spectra data were processed with a multidimensional projection technique. The robustness of the immunosensors in dealing with patient samples without suffering interference from analytes present in biological fluid...Continue Reading

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May 21, 2015·ACS Applied Materials & Interfaces·Juliana Coatrini SoaresOsvaldo N Oliveira
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Mar 5, 2016·Physical Chemistry Chemical Physics : PCCP·Juliana Coatrini SoaresOsvaldo N Oliveira
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Citations

Jun 7, 2020·Micromachines·Wen-Chi YangCongo Tak-Shing Ching
Aug 25, 2020·Biomaterials Science·Marta CavoLoretta L Del Mercato
Apr 27, 2019·Nanomaterials·Rafael Contreras-CáceresJose Prados
Feb 2, 2019·Analytical and Bioanalytical Chemistry·Nongnoot Wongkaew
Jul 4, 2020·Current Drug Targets·Pablo RedruelloJose Prados
Aug 8, 2021·International Journal of Molecular Sciences·Andreea NedelcuLucian Mocan
Aug 6, 2021·Nanomedicine·Laura Russo
Nov 26, 2019·ACS Applied Materials & Interfaces·Juliana Coatrini SoaresOsvaldo N Oliveira
Sep 17, 2020·ACS Applied Materials & Interfaces·Danilo M Dos SantosLuiz H C Mattoso

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
biosensors
biosensor
scanning electron microscopy

Software Mentioned

IDMAP
Interactive Document Map ( IDMAP

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