Immunosensor for the ultrasensitive and quantitative detection of bladder cancer in point of care testing

Biosensors & Bioelectronics
Cheng-Hsin ChuangMuhammad Omar Shaikh

Abstract

An ultrasensitive and real-time impedance based immunosensor has been fabricated for the quantitative detection of Galectin-1 (Gal-1) protein, a biomarker for the onset of multiple oncological conditions, especially bladder cancer. The chip consists of a gold annular interdigitated microelectrode array (3×3 format with a sensing area of 200µm) patterned using standard microfabrication processes, with the ability to electrically address each electrode individually. To improve sensitivity and immobilization efficiency, we have utilized nanoprobes (Gal-1 antibodies conjugated to alumina nanoparticles through silane modification) that are trapped on the microelectrode surface using programmable dielectrophoretic manipulations. The limit of detection of the immunosensor for Gal-1 protein is 0.0078mg/ml of T24 (Grade III) cell lysate in phosphate buffered saline, artificial urine and human urine samples. The normalized impedance variations show a linear dependence on the concentration of cell lysate present while specificity is demonstrated by comparing the immunosensor response for two different grades of bladder cancer cell lysates. We have also designed a portable impedance analyzing device to connect the immunosensor for regular ...Continue Reading

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Citations

Feb 23, 2018·The Analyst·Puja Sandbhor Gaikwad, Rinti Banerjee
Mar 3, 2018·The Analyst·Dan LiuChaoyong Yang
Jun 12, 2020·Diagnostics·Kit Man ChanMelanie MacGregor
Jul 6, 2019·Biomedical Microdevices·M Anne Sawhney, R S Conlan
Jul 2, 2020·Biomedical Microdevices·Muhammad Omar ShaikhCheng-Hsin Chuang
Sep 15, 2018·International Journal of Molecular Sciences·Joshua M CampbellAdam T Melvin
Aug 14, 2020·ACS Combinatorial Science·Ina Turcan, Marius Andrei Olariu
Jan 21, 2017·Analytical Chemistry·Wei WenYuehe Lin
Jun 23, 2020·ACS Omega·Rawdha DekhiliJolanda Spadavecchia

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