Immunosignals of Oligodendrocyte Markers and Myelin-Associated Proteins Are Critically Affected after Experimental Stroke in Wild-Type and Alzheimer Modeling Mice of Different Ages

Frontiers in Cellular Neuroscience
D MichalskiWolfgang Härtig

Abstract

Because stroke therapies are still limited and patients are often concerned by long-term sequelae with significant impairment of daily living, elaborated neuroprotective strategies are needed. During the last decades, research substantially improved the knowledge on cellular pathologies responsible for stroke-related tissue damage. In this context, the neurovascular unit (NVU) concept has been established, summarizing the affections of neurons, associated astrocytes and the vasculature. Although oligodendrocytes were already identified to play a major role in other brain pathologies, their role during stroke evolution and long-lasting tissue damage is poorly understood. This study aims to explore oligodendrocyte structures, i.e., oligodendrocytes and their myelin-associated proteins, after experimental focal cerebral ischemia. For translational issues, different ages and genotypes including an Alzheimer-like background were considered to mimic potential co-morbidities. Three- and 12-month-old wild-type and triple-transgenic mice were subjected to unilateral middle cerebral artery occlusion. Immunofluorescence labeling was performed on forebrain tissues affected by 24 h of ischemia to visualize the oligodendrocyte-specific prote...Continue Reading

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Citations

Mar 17, 2020·Frontiers in Immunology·Shenbin XuJianmin Zhang
Jan 4, 2019·Acta Veterinaria Scandinavica·Sandra SchönigerWolfgang Härtig
Apr 23, 2020·Frontiers in Neurology·Zhijuan CaoGary K Steinberg
Oct 18, 2020·International Journal of Molecular Sciences·Ciro De LucaAnna Maria Colangelo

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Methods Mentioned

BETA
electron microscopy
transgenic

Software Mentioned

BZ II analyzer
Microsoft PowerPoint
SPSS
BZ II viewer

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