PMID: 301553Aug 1, 1977

Immunostimulators induce granulocyte/macrophage colony-stimulating activity and block proliferation in a monocyte tumor cell line

The Journal of Experimental Medicine
P RalphI Nakoinz

Abstract

Monocyte tumor cell line PU5-1.8 does not normally produce colony-stimulating activity (CSA) required by granulocyte and macrophage progenitors to proliferate and mature in agar. However, CSA is induced in the culture line by as little as 10 ng/ml endotoxic lipopolysaccharide (LPS), with maximum CSA production and release to the medium between 2 and 3 days of incubation. Derived lipid A, but not alkali-treated LPS, is also active. Induction requires RNA and protein synthesis, but is not blocked by mitomycin C or Colcemid. Other inducers of CSA include Mycobacterium Bacillus Calmette-Guérin, tuberculin protein preparation purified protein derivative, zymosan, and phorbol myristate. All inducing agents are specific inhibitors of the monocyte tumor cell proliferation in vitro. Latex beads, another macrophage-activating agent, are rapidly phagocytosed by PU5-1.8 cells, but neither inhibit growth nor induce CSA.

Citations

Jan 1, 1980·International Journal of Immunopharmacology·J A Hamilton, M A Moore
Dec 1, 1985·Scandinavian Journal of Immunology·M J Dauphinée, N Talal
Jan 1, 1979·Immunological Reviews·P Ralph
Nov 20, 2012·Oncoimmunology·Erika VacchelliGuido Kroemer
Mar 1, 1981·Journal of Cellular Physiology·J A Hamilton, S R Dientsman
Jan 1, 1987·Journal of Cellular Physiology·K Ohki, A Nagayama

Related Concepts

Cell Division Phases
Protein Inducer MGI
Neoglycoproteins
Granulocyte
Leukocytes
Lipopolysaccharides
Macrophage
Monocytes
Malignant Neoplasms

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