PMID: 25779661Mar 18, 2015Paper

Immunostimulatory effects of RACK1 pseudosubstrate in human leukocytes obtained from young and old donors

Oncotarget
Emanuela CorsiniMarco Racchi

Abstract

Aims of this study were to investigate the ability of RACK1 pseudosubstrate alone or in combination with classical immune stimuli to activate human leukocytes, and to restore age-associated immune defects.A total of 25 donors (17 old donors, 77-79 yrs; 8 young donors, 25-34 yrs) were enrolled. To evaluate the effect of RACK1 pseudosubstrate on cytokine production and CD86 expression the whole blood assay was used. Cultures were treated with RACK1 pseudosubstrate in the presence or absence of lipopolysaccharide (LPS) or phytohaemagglutinin (PHA) and incubated for 24 h or 48 h for LPS-induced CD86 expression, TNF-α, IL-6, IL-8, IL-10 production, and PHA-induced IL-4, IL-10, IFN-γ, respectively. RACK1 pseudosubstrate alone induced IL-6, IL-8, and CD86 expression in both young and old donors, and IFN-γ in old donors. In combination with LPS an increase in IL-8, IL-10 and TNF-α was observed, also resulting in restoration of age-associated defective production, while no changes in the other parameters investigated were found.Even if based on a small sample size, these results suggest the possibility to by-pass some of age-associated immune alterations, which may be beneficial in situations were natural immune stimulation is required,...Continue Reading

References

Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·D Ron, D Mochly-Rosen
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·D RonD Mochly-Rosen
Aug 9, 1996·Science·M LeitgesA Tarakhovsky
Jul 11, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·A D DelpedroO Bakouche
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·J M PassP Ping
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emanuela CorsiniCorrado L Galli
Nov 19, 2002·Molecular Pharmacology·Angela McCahillStephen J Yarwood
May 11, 2005·Immunological Reviews·Graham PawelecAnders Wikby
Jun 23, 2009·Trends in Immunology·Alexander PandaAlbert C Shaw
Nov 26, 2009·American Journal of Physiology. Endocrinology and Metabolism·Alexandra C Newton
Mar 29, 2012·Advances in Experimental Medicine and Biology·Lily ZengPhilip M Newton
Oct 31, 2012·International Journal of Molecular Sciences·Daniela Cosentino-GomesJosé Roberto Meyer-Fernandes
Feb 23, 2013·Trends in Immunology·Kok-Fai Kong, Amnon Altman
Mar 28, 2013·BMC Research Notes·Anbarasu DeenadayalanAlamelu Raja
Jun 26, 2013·Expert Opinion on Therapeutic Patents·Masilamani Elizabeth SobhiaAlekhya Kokkula
Aug 7, 2013·Cell Communication and Signaling : CCS·Dorit RonPatrick A Kiely
Oct 19, 2013·Archives of Toxicology·Emanuela CorsiniCorrado L Galli
Nov 1, 2013·Medical Oncology·Julhash U KaziLars Rönnstrand
Jan 21, 2014·Biochemical Pharmacology·Hueng-Chuen FanJenn-Haung Lai
May 17, 2014·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Claudio Franceschi, Judith Campisi
Jun 18, 2014·Current Opinion in Immunology·Tamas FulopAnis Larbi

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Citations

Jul 27, 2018·The Tohoku Journal of Experimental Medicine·Xiaoxin ZhangPing Xue
May 15, 2016·Immunologic Research·Emanuela CorsiniMarco Racchi

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