Immunostimulatory sequence up-modulate the interaction between immunoglobulins and plasmid DNA at acidic pH

Vaccine
H ZhenlinZ Fengjuan

Abstract

To identify specific receptors for immunostimulatory sequence (ISS), a cDNA library of human bone marrow was screened by one-hybrid system using ISS as bait. Among four duel-positive clones isolated so far, two encode light chain of immunoglobulin (Ig) homologous to anti-DNA and anti-HBsAg (HBsAb), respectively, suggesting a direct interaction between ISS and Igs. ELISA revealed that HBsAb, as well as Igs in normal mouse serum and a monoclonal anti-v5 antibody, bound to plasmid DNA in a pH-dependent way; more importantly, the plasmids inserted with more copies of ISS shown higher reactivity with these Igs than their blank vectors. Electrophoretic mobility shift assay (EMSA) showed HBsAb bound to oligonucleotides (ODN) also in a pH-dependent and ISS-specific way. Furthermore, ISS-containing ODN could enhance the binding of Igs to plasmid DNA at acidic pH. These results support existence of direct ISS-Ig interaction and demonstrated that DNA-Ig interaction could be up-modulated by ISS. In consideration of the crucial role of membrane Ig (mIg) in B cell activation, these results suggest mIg may be a candidate ISS receptor in ISS-induced B cell activation.

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L A YakubovV V Vlassov
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L LokeL M Neckers
Apr 6, 1995·Nature·A M KriegD M Klinman
Dec 1, 1995·Trends in Biochemical Sciences·J B AllenS J Elledge
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·K RajagopalanH Kohler
Aug 1, 1996·The Journal of Laboratory and Clinical Medicine·A M Krieg
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·A M KriegH L Davis
Apr 9, 1999·Vaccine·E Y RykovaV V Vlassov
Mar 11, 2000·Immunology·S BehboudiJ Austyn

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.