Immunosuppressant drug tacrolimus induced mitochondrial nephrotoxicity, modified PCNA and Bcl-2 expression attenuated by Ocimum basilicum L. in CD1 mice

Toxicology Reports
Atif Abdulwahab A OyouniHasibur Rehman

Abstract

Tacrolimus (TAC) is used sporadically as an immunosuppressive agent for organ transplantation, but its clinical used is limited due to its marked nephrotoxicity. Ocimum basilicum L. (Lamiaceae) (OB) had been shown to possess antioxidant, anti-inflammatory and nephroprotective activity, and effective at improving renal inflammation and glomerular. In our study, we aim to evaluate the efficacy of the OB against TAC-induced mitochondrial nephrotoxicity in CD1 mice. Mice were randomly divided into four groups. Group 1 (control group); administered orally with normal saline (1 mL/kg) for two weeks; Group 2 (OB extract treated-group) (500 mg/kg b.wt) gavaged once/day for two weeks; Group 3 (TAC-treated group) (3 mg/kg b.wt, administered ip once a day for two weeks); and Group 4; (TAC plus OB extract treated-group). Tacrolimus-induced nephrotoxicity was assessed biochemically and histopathologically. The OB extract was high in phenolic content (50.3 mg/g of gallic acid equivalent), total flavonoids (14.5 mg/g CE equivalent). The potential antioxidant efficacy of the extract (IC50) was 24.5 μg/mL. OB pretreatment significantly improved the TAC-induced changes in biochemical markers of nephrotoxicity for instance blood urea nitrogen (BU...Continue Reading

Citations

Jan 10, 2020·Environmental Science and Pollution Research International·Ehab ToussonThulfiqar Fawwaz Mutar
Aug 9, 2020·Oxidative Medicine and Cellular Longevity·Maha A AldubayanRehab M Elgharabawy
Nov 17, 2020·Oxidative Medicine and Cellular Longevity·Shatha G FelembanIbtesam S Almami
Apr 15, 2021·Environmental Science and Pollution Research International·Mabrouk Attia Abd EldaimHebatalla Nashaat Elsharkawy
May 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mabrouk Attia Abd EldaimHebatalla Nashaat Elsharkawy

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