PMID: 8614067May 1, 1996Paper

Immunosuppression after endotoxin shock: the result of multiple anti-inflammatory factors

The Journal of Trauma
W G JungerR Coimbra

Abstract

Endotoxin induced suppression of cellular immune function is thought to contribute to septic complications in trauma patients. A rabbit model of endotoxemia was used to determine the relative roles of the anti-inflammatory factors interleukin-4 (IL-4), interleukin-10 (IL-10), transforming growth factor beta1 (TGFbeta1), and prostaglandin E2 (PGE2) in addition to other factors, in inducing immunosuppression. T-cell suppressive factors (TSF) in serum ultrafiltrates were separated and tested for the presence of the known suppressive factors PGE2, IL-4, IL-10, and TGFbeta1. New Zealand rabbits were injected with 50 microg/kg of purified Escherichia coli lipopolysaccharide. Animals were exsanguinated after 48 hours and serum was separated by ultrafiltration (cutoff 50 kd), TSK HW-40 size exclusion chromatography, and Q-Sepharose anion exchange chromatography. TSF activities of chromatographic fractions and serum samples were measured with a mitogen induced in vitro T-cell proliferation assay. Levels of PGE2, IL-4, IL-10, and TGFbeta1 were measured with enzyme immunoassays. Serum TSF activity, and levels of PGE2, IL-4, IL-10, and TGFbeta1 were increased after endotoxemia. Size exclusion chromatography revealed three major fractions (...Continue Reading

References

Mar 1, 1991·Journal of Clinical Immunology·C L Miller-GrazianoD Catalano
Feb 1, 1991·Archives of Surgery·M D EpsteinJ W Vester
Mar 1, 1988·Archives of Surgery·C L Miller-GrazianoK Kodys
Dec 1, 1988·The Journal of Trauma·G X XiaoR A Winchurch
Feb 1, 1988·American Journal of Surgery·A J SoriG W Machiedo
Apr 1, 1988·The Journal of Trauma·D B HoytJ B Fortune
May 1, 1988·The Journal of Trauma·A N OzkanJ J Sullivan
Apr 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·F E Stiekema, M L Kapsenberg
May 1, 1981·Prostaglandins·H G MorrisF T Shepperdson

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Citations

Feb 15, 2001·Critical Care Medicine·T SfeirE C Rackow
Oct 26, 2005·BMC Surgery·Carsten J KronesVolker Schumpelick
Aug 15, 2001·American Journal of Physiology. Cell Physiology·W H LoomisW G Junger
Sep 7, 2016·PloS One·Fabrício Freitas FernandesAdemilson Panunto-Castelo
Apr 12, 2020·Journal of Clinical Medicine·Ramona SturmBorna Relja
Aug 26, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K VargaG Kunos
Jul 13, 2006·Annual Review of Biomedical Engineering·Geert W Schmid-Schönbein
Jul 16, 2013·Mediators of Inflammation·Wibke SchulteRichard Bucala
Jan 24, 2015·Mediators of Inflammation·Juliana Reis MachadoMara Rúbia Nunes Celes
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hua LiuTong Cao
Jun 9, 2005·Critical Care Medicine·Peter A Ward
Apr 5, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Geert W Schmid-Schönbein, Tony E Hugli

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