Immunosuppressive drugs affect high-mannose/hybrid N-glycans on human allostimulated leukocytes

Analytical Cellular Pathology (Amsterdam)
Ewa PochećAnna Lityńska

Abstract

N-glycosylation plays an important role in the majority of physiological and pathological processes occurring in the immune system. Alteration of the type and abundance of glycans is an element of lymphocyte differentiation; it is also common in the development of immune-mediated inflammatory diseases. The N-glycosylation process is very sensitive to different environmental agents, among them the pharmacological environment of immunosuppressive drugs. Some results show that high-mannose oligosaccharides have the ability to suppress different stages of the immune response. We evaluated the effects of cyclosporin A (CsA) and rapamycin (Rapa) on high-mannose/hybrid-type glycosylation in human leukocytes activated in a two-way mixed leukocyte reaction (MLR). CsA significantly reduced the number of leukocytes covered by high-mannose/hybrid N-glycans, and the synergistic action of CsA and Rapa led to an increase of these structures on the remaining leukocytes. This is the first study indicating that β1 and β3 integrins bearing high-mannose/hybrid structures are affected by Rapa and CsA. Rapa taken separately and together with CsA changed the expression of β1 and β3 integrins and, by regulating the protein amount, increased the oligom...Continue Reading

References

Mar 20, 1999·Transplantation Proceedings·L C PaulS Zhang
Oct 26, 1999·Journal of Molecular Biology·P M RuddI A Wilson
May 10, 2000·The Journal of Biological Chemistry·E S HarrisG A Zimmerman
Jul 6, 2000·Immunopharmacology·A C Allison, E M Eugui
Jan 3, 2001·Kidney International·R N SaundersM L Nicholson
Jun 19, 2001·Current Opinion in Immunology·M L Dustin, A R de Fougerolles
Jun 26, 2001·Glycoconjugate Journal·A Kobata
Sep 18, 2002·Immunological Reviews·Tasha N Sims, Michael L Dustin
Sep 28, 2002·Nature Immunology·Mark A DanielsStephen C Jameson
Apr 12, 2003·The Journal of Biological Chemistry·Marie-Agnès DouceyImmanuel F Luescher
Oct 3, 2003·Journal of Pediatric Surgery·Marion M Aw
Nov 6, 2003·Journal of Cell Science·Nancy HoggAlison McDowall
Mar 23, 2004·Annual Review of Immunology·Jonathan T PribilaYoji Shimizu
Jul 2, 2004·Molecular and Cellular Biochemistry·Ying ZhangHui-li Chen
Sep 29, 2004·Trends in Biotechnology·Pauline M RuddRaymond A Dwek
Jun 3, 2005·Nature Reviews. Drug Discovery·Danielle H Dube, Carolyn R Bertozzi
Dec 22, 2005·Nature Immunology·Andrew D LusterUlrich H von Andrian
Sep 9, 2006·Cell·Kazuaki Ohtsubo, Jamey D Marth
Mar 16, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A Lacy-HulbertR Abdi
Jul 13, 2007·Immunological Reviews·David M RoseMark H Ginsberg
May 21, 2008·Nature Immunology·Yvette van Kooyk, Gabriel A Rabinovich
Aug 30, 2008·Immunology and Cell Biology·Stuart M HaslamAnne Dell
Oct 11, 2008·Nature Reviews. Immunology·Jamey D Marth, Prabhjit K Grewal
Jan 2, 2009·Journal of Cell Science·Rachel EvansNancy Hogg
Mar 6, 2009·Cell Adhesion & Migration·Jianguo Gu, Naoyuki Taniguchi
May 6, 2009·The Journal of Biological Chemistry·Tiffany Frey, Antonio De Maio
Jul 15, 2009·Immunological Reviews·Markus SperandioKlaus Ley
Nov 12, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Katarzyna BocianGrazyna Korczak-Kowalska
Mar 5, 2010·The Journal of Allergy and Clinical Immunology·Javier Chinen, Rebecca H Buckley
May 5, 2010·The Journal of Cell Biology·Gillian M GriffithsJane C Stinchcombe
May 28, 2011·Current Opinion in Cell Biology·Cecile BoscherIvan R Nabi
Jan 4, 2012·Immunology·Yanbo Zhang, Hongyan Wang
Feb 1, 2012·Annals of the New York Academy of Sciences·Mary C Clark, Linda G Baum
Mar 31, 2012·Seminars in Immunopathology·Sean O Ryan, Brian A Cobb
Apr 25, 2012·Annals of the New York Academy of Sciences·Gabriel A RabinovichBrian A Cobb
Mar 15, 2013·Trends in Immunology·Jenny L JohnsonBrian A Cobb
Oct 9, 2013·Biochimica Et Biophysica Acta·Jennifer A FaralliDonna M Peters
Jun 5, 2014·The Journal of Biological Chemistry·Tomoya IsajiJianguo Gu
Oct 21, 2014·Drug Design, Development and Therapy·Karolina KędzierskaKazimierz Ciechanowski

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Citations

Oct 12, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guowei ZhangJianguo Gu

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Methods Mentioned

BETA
glycosylation
density-gradient centrifugation
flow cytometry
xenograft

Software Mentioned

Mascot Distiller
UVImap Image Quantification
Cell Quest
MatrixScience

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