Immunosuppressive effects of the standardized extract of Phyllanthus amarus on cellular immune responses in Wistar-Kyoto rats

Drug Design, Development and Therapy
Menaga IlangkovanSyed Nasir Abbas Bukhari

Abstract

Phyllanthus amarus (family: Euphorbiaceae) is of immense interest due to its wide spectrum of biological activities. In the present study, the standardized 80% ethanol extract of P. amarus was investigated for its modulatory activity on various cellular immune parameters, including chemotaxis of neutrophils, engulfment of Escherichia coli by neutrophils, and Mac-1 expression, in leukocytes isolated from treated/nontreated Wistar-Kyoto rats. The detailed cell-mediated activity of P. amarus was also investigated, including analysis of the effects on T- and B-cell proliferation and CD4(+) and CD8(+) T-cell subsets in splenic mononuclear cells, and estimation of serum cytokine production by activated T-cells. The main components of the extract, phyllanthin, hypophyllanthin, corilagin, geraniin, ellagic acid, and gallic acid were identified and quantitatively analyzed in the extracts, using validated reversed-phase high-performance liquid chromatography (HPLC) methods. N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced neutrophils isolated from rats administered with the extract of P. amarus, at doses ranging from 100 to 400 mg/kg for 14 days, revealed a significant dose-dependent reduction in neutrophil migration (P<0.05). Simi...Continue Reading

Citations

May 4, 2016·Phytotherapy Research : PTR·Menaga IlangkovanSyed Nasir Abbas Bukhari
Jan 9, 2019·Phytotherapy Research : PTR·Nor Shazliana GhazaleeMd Areeful Haque
Jul 3, 2020·BMC Complementary Medicine and Therapies·Elysha Nur IsmailNorazrina Azmi

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Datasets Mentioned

BETA
UKMB
30078

Methods Mentioned

BETA
flow cytometry
Assay
dissection

Software Mentioned

Cell Quest Pro
GraphPad Prism
FACScan

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