PMID: 3768424Nov 19, 1986Paper

Immunotargeting of erythrocyte-bound streptokinase provides local lysis of a fibrin clot

Biochimica Et Biophysica Acta
Vladimir R MuzykantovV N Smirnov

Abstract

The creation of an anticollagen antibody-erythrocyte-streptokinase complex has been described. Immobilization of both proteins on erythrocyte membrane has been performed using an avidin-biotin interaction. Modification of streptokinase with (6-biotinylamido)hexanoic acid N-hydroxysuccinimide ester at the concentration of 1.1 mM (20% modification of protein amino groups) provides effective (up to 90%) attachment of streptokinase to an avidin-carrying erythrocyte surface. The loss of streptokinase activity due to modification under these conditions is not significant. The maximal attachment of streptokinase was equal to about 50 ng per 10(6) erythrocytes, i.e., about 5 X 10(5) molecules of streptokinase per erythrocyte. The presence of streptokinase in the incubation mixture inhibited the attachment of antibodies by about 50%. Nevertheless, co-immobilization of anticollagen antibody (1.0 X 10(5) molecules per cell) and streptokinase (2.8 X 10(5) molecules per cell) on the erythrocyte surface provided firm and specific binding of such erythrocytes to a collagen-coated surface (1.6 X 10(6) bound cells per 1 cm2 on a collagen-coated surface against 0.006 X 10(6) bound cells on a bovine serum albumin-coated surface). Targeting of suc...Continue Reading

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Citations

May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·V R MuzykantovA B Fisher
Nov 10, 2001·Annual Review of Biomedical Engineering·S L Diamond
Apr 2, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rajesh MukthavaramDmitri Simberg
Oct 31, 2016·Advanced Drug Delivery Reviews·Carlos H VillaVladimir Muzykantov
Nov 13, 2007·Advanced Drug Delivery Reviews·F PierigèM Magnani
Jun 10, 2014·Expert Opinion on Drug Delivery·Manisha BhateriaRabi Sankar Bhatta
Nov 28, 2012·Expert Opinion on Drug Delivery·Vladimir R Muzykantov
Aug 1, 2015·Therapeutic Delivery·Carlos H VillaVladimir R Muzykantov
Jun 27, 2013·Blood·Colin F GreinederVladimir R Muzykantov
Oct 7, 2016·Transfusion Medicine Reviews·Carlos H VillaVladimir Muzykantov
Mar 3, 2010·Expert Opinion on Drug Delivery·Vladimir R Muzykantov
Nov 22, 2005·Expert Opinion on Drug Delivery·Luiga RossiMauro Magnani
Dec 4, 1992·Annals of the New York Academy of Sciences·R Fears
May 14, 2020·Pharmaceutics·Bridget E Bax
Sep 15, 2020·Expert Opinion on Drug Delivery·Luigia RossiMauro Magnani
Apr 26, 2021·Drug Discovery Today·Lei ZhangZhe-Sheng Chen
Jan 1, 1997·Blood Cells, Molecules & Diseases·A Krantz
Jun 30, 1993·Biochimica Et Biophysica Acta·V R MuzykantovE K Ruuge
Apr 1, 2021·Annual Review of Biomedical Engineering·Jacob S BrennerVladimir R Muzykantov

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