Abstract
Polysaccharide nucleic acid fractions of bacillus Calmette-Guérin, termed BCG-PSN, have traditionally been used as immunomodulators in the treatment of dermatitis and allergic diseases. While the sales of injectable BCG-PSN have shown steady growth in recent years, no reports of using BCG-PSN powder or its immunotherapeutic effects exist. Here, BCG-PSN powder was applied directly to the skin to evaluate the immunotherapeutic effects on mice infected with Mycobacterium tuberculosis (MTB). In total, 34 μg of BCG-PSN powder could be loaded into a microneedle patch (MNP). Mice receiving BCG-PSN powder delivered via MNP exhibited significantly increased IFN-γ and TNF-α production in peripheral blood CD4 + T cells and improved pathological changes in their lungs and spleens compared to control group mice. The immunotherapeutic effect of BCG-PSN powder delivered via MNP was better than that delivered via intramuscular injection to some extent. Furthermore, MNPs eliminate the side effects of syringes, and this study demonstrated that BCG-PSN can be clinically administrated in powder form.
References
Dec 1, 1993·The Journal of Experimental Medicine·J L FlynnB R Bloom
Jul 1, 1997·Journal of Internal Medicine·J R FraserU B Laurent
Jul 19, 2003·The Journal of Infectious Diseases·Sonal S MunsiffBarry N Kreiswirth
Mar 25, 2004·Nature Reviews. Immunology·Thomas S Kupper, Robert C Fuhlbrigge
Jul 24, 2004·Clinical and Experimental Immunology·B M SaundersW J Britton
Oct 22, 2005·Nature Reviews. Immunology·Samir Mitragotri
Aug 22, 2008·The Clinical Journal of Pain·Harvinder S GillMark R Prausnitz
Jun 3, 2009·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·C XiongG Zhou
Mar 20, 2010·Drug Delivery·Ryan F DonnellyA David Woolfson
Feb 1, 2011·Vaccine·Yasuhiro HiraishiMark R Prausnitz
Jul 9, 2011·Vaccine·Lissete RodriguezArmando Acosta
May 12, 2012·Advanced Drug Delivery Reviews·Yeu-Chun KimMark R Prausnitz
Nov 24, 2012·Vaccine·Jing SunYiming Shao
Mar 1, 2008·Advanced Materials·Sean P SullivanMark R Prausnitz
Apr 15, 2014·Clinical Epidemiology·Alberto MatteelliAnna Cc Carvalho
May 9, 2014·Journal of Internal Medicine·S K ParidaM Maeurer
Jun 4, 2014·Clinical Medicine : Journal of the Royal College of Physicians of London·Gunar Günther
Aug 20, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xinyuan ChenMei X Wu
Dec 3, 2014·Asian Pacific Journal of Tropical Medicine·Liu-Hui WangTao Xiao
Feb 12, 2015·Human Vaccines & Immunotherapeutics·Yanely TiradoArmando Acosta
Apr 11, 2015·Carbohydrate Polymers·Dongfeng WeiLixia Yuan
May 26, 2015·Carbohydrate Polymers·Xiongbo XuXiu Xin
May 29, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mudnakudu Nagaraju Kiran KumarMei X Wu
Jun 18, 2016·Carbohydrate Polymers·Wei LiuXiangdong Gao
Apr 10, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fan ChenMei X Wu
Citations
Mar 31, 2018·Human Vaccines & Immunotherapeutics·Wenping GongXueqiong Wu
Aug 2, 2018·Bioengineering·Chantell FariasMaryam Mobed-Miremadi
Jul 31, 2020·Drug Delivery·Qinying YanGensheng Yang
Mar 21, 2019·Drug Delivery and Translational Research·Wei WangFan Chen
Oct 9, 2020·Vaccines·E WhitlowS N M Hanif
Dec 20, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hamed AmaniHélder A Santos
May 1, 2021·Vaccines·Jesse O'SheaNadine Rouphael