Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice

Plant Biotechnology Journal
James A GregoryM Cecilia Berin

Abstract

Peanut allergy is an IgE-mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates immunotherapy studies. Moreover, peanut immunotherapy is associated with frequent negative side effects and patients are often at risk of allergic reactions once immunotherapy is discontinued. Allergen-specific approaches using recombinant proteins are an attractive alternative because they allow more precise dosing and the opportunity to engineer proteins with improved safety profiles. We tested whether Ara h 1 and Ara h 2, two major peanut allergens, could be produced using chloroplast of the unicellular eukaryotic alga, Chlamydomonas reinhardtii. C. reinhardtii is novel host for producing allergens that is genetically tractable, inexpensive and easy to grow, and is able to produce more complex proteins than bacterial hosts. Compared to the native proteins, algal-produced Ara h 1 core domain and Ara h 2 have a reduced affinity for IgE from peanut-allergic patients. We further found that immunotherapy using algal-p...Continue Reading

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Citations

May 4, 2017·International Archives of Allergy and Immunology·Angelika Tscheppe, Heimo Breiteneder
Jan 26, 2018·Clinical Reviews in Allergy & Immunology·Quindelyn S Cook, A Wesley Burks
Jun 10, 2020·Critical Reviews in Biotechnology·Jeppe Madura LarsenKatrine Lindholm Bøgh
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Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sergio Rosales-MendozaSornkanok Vimolmangkang
May 12, 2017·International Archives of Allergy and Immunology·Sonja HirschlMichael Wallner

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Datasets Mentioned

BETA
AAN77576

Methods Mentioned

BETA
transgenic
PCR
ELISA
flow cytometry
phage display
glycosylation
acetylation

Software Mentioned

GraphPad Prism
Gene Designer
GeneWiz

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