Immunotherapy with autologous tumor cells engineered to secrete Tag7/PGRP, an innate immunity recognition molecule

The Journal of Gene Medicine
Sergey S LarinSergei L Kiselev

Abstract

Recent studies indicate that the innate component of immune defense plays an important role in the establishment of antigen-specific immune response. We have previously isolated a novel mouse gene tag7/PGRP that was shown to be involved in the innate component of the immune system, and its insect homologue is an upstream mediator of Toll signaling in Drosophila. Transiently or stably genetically modified mouse tumor cell lines expressing Tag7 were used. Tumor growth rate and animal survival were analyzed. Possible effector cells involved in tumor suppression were detected immunohistochemically. Transfection of mammary gland adenocarcinoma cells with the tag7 cDNA did not alter their growth rate in vitro but diminished their tumorogenicity in vivo in syngeneic and immunodeficient animals. Increased incidence of apoptosis was registered in the modified tumors. Transient expression of Tag7 by mouse melanoma M3 cells elicited protective immunity against parental tumor cells. Immunohistochemical analysis revealed that tumors after immunization with the genetically modified cells were infiltrated with Mac1(+) cells, B220(+) cells, and NK cells. Using nude mice we observed rejection of modified cells, but did not detect memory formati...Continue Reading

Citations

Jan 5, 2014·Doklady. Biochemistry and Biophysics·L O SkorodumovaS S Larin
Aug 3, 2007·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·E A DukhaninaN V Gnuchev
May 23, 2007·Molekuliarnaia biologiia·S A Nedospasov, D V Kuprash
Mar 30, 2016·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·O K IvanovaD V Yashin
Dec 12, 2018·IUBMB Life·Tatiana N SharapovaDenis V Yashin
Sep 30, 2004·Gene Therapy·S S LarinS L Kiselev
Jul 18, 2015·The Journal of Biological Chemistry·Denis V YashinLidia P Sashchenko
Jun 1, 2018·Journal of Immunology Research·T N SharapovaD V Yashin
Jan 22, 2021·Nucleic Acids Research·Ksenia S EgorovaValentine P Ananikov

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