Abstract
The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immunotherapy (SCIT) using a murine model of peach allergy. After sensitization with Pru p 3, BALB/c mice received SCIT with Pru p 3 or R/A Pru p 3 and were challenged with Pru p 3. SCIT with Pru p 3, but not with R/A Pru p 3, suppressed anaphylaxis upon the challenge significantly. SCIT with Pru p 3 did not suppress Pru p 3-specific IgE and IgG1 production, but enhanced IgG2a production. In contrast, SCIT with R/A Pru p 3 suppressed IgE and IgG1 production, but enhanced IgG2a production only moderately. The therapeutic efficacy of SCIT with Pru p 3 was associated with induction of IL-10 and IFN-γ. Hypoallergenic folding variant of Pru p 3 is not likely an efficacious therapeutic component in SCIT of peach allergy. The lower efficacy of R/A Pru p 3 might be attributed to poor antigenicity and/or weak stability due to its unfolded conformation.
References
May 1, 1979·British Journal of Pharmacology·J A ParsonsJ M Zanelli
Jul 11, 2000·The Journal of Allergy and Clinical Immunology·X M LiH A Sampson
Nov 25, 2003·Current Opinion in Immunology·Stephanie C EisenbarthKim Bottomly
Jun 18, 2005·Memórias do Instituto Oswaldo Cruz·Leonardo K TeixeiraJoão P B Viola
Mar 25, 2006·Immunology·Alison TaylorCezmi A Akdis
Aug 8, 2006·The Journal of Allergy and Clinical Immunology·Emilia VassilopoulouClare Mills
May 10, 2007·Current Opinion in Allergy and Clinical Immunology·Laurian Zuidmeer, Ronald van Ree
Feb 11, 2009·The Journal of Allergy and Clinical Immunology·Adam T FoxGideon Lack
Aug 12, 2009·The Journal of Experimental Medicine·Nicole SchmitzMartin F Bachmann
Jun 19, 2010·International Archives of Allergy and Immunology·Christina HartzStephan Scheurer
Sep 2, 2010·Allergy·T SaarneM van Hage
May 17, 2011·The Journal of Allergy and Clinical Immunology·Masako TodaStephan Scheurer
Mar 14, 2012·Clinical and Translational Allergy·Laurian Zuidmeer-JongejanRonald van Ree
Feb 22, 2013·Current Opinion in Allergy and Clinical Immunology·Riccardo Asero, Valerio Pravettoni
Jan 22, 2014·Nature Reviews. Immunology·Martin GuilliamsBart N Lambrecht
Mar 4, 2014·The Journal of Allergy and Clinical Immunology·Mübeccel Akdis, Cezmi A Akdis
Oct 3, 2014·Clinical and Translational Allergy·Katherine Anagnostou, Andrew Clark
Nov 20, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Ahlem BouazizMarie-Eve Dumez
Dec 6, 2014·Allergy·D M FonsecaV L D Bonato
Aug 26, 2015·Allergy·S SiebeneicherM Toda
Nov 13, 2015·Expert Review of Clinical Immunology·Laurian JongejanLars K Poulsen
Feb 9, 2016·The Journal of Allergy and Clinical Immunology·Rudolf ValentaVerena Niederberger
Nov 24, 2016·The Journal of Allergy and Clinical Immunology·Raphaela FreidlBirgit Linhart
Jan 7, 2017·Allergy·U NurmatovA Sheikh
Jan 14, 2017·Scientific Reports·Maria J RodriguezCristobalina Mayorga
Jun 7, 2017·Molecular Nutrition & Food Research·Maria J RodriguezCristobalina Mayorga
Citations
Nov 21, 2018·Molecular Nutrition & Food Research·Maria Jose RodriguezJames Richard Perkins
Jun 18, 2019·Molecular Nutrition & Food Research·Stephanie EichhornGabriele Gadermaier
Jun 3, 2021·Foods·Cristobalina MayorgaFrancisca Gómez
Dec 28, 2019·Current Pediatric Reviews·Fernanda ChieraGiovanni Battista Pajno