Immunotherapy with Native Molecule rather than Hypoallergenic Variant of Pru p 3, the Major Peach Allergen, Shows Beneficial Effects in Mice

Journal of Immunology Research
Maria J RodriguezCristobalina Mayorga

Abstract

The use of hypoallergenic derivatives is considered beneficial to promote the safety and efficacy of allergen-specific immunotherapy. We aimed to assess the efficacy of reduced and alkylated (R/A) Pru p 3, a hypoallergenic folding variant of the major peach allergen, in subcutaneous immunotherapy (SCIT) using a murine model of peach allergy. After sensitization with Pru p 3, BALB/c mice received SCIT with Pru p 3 or R/A Pru p 3 and were challenged with Pru p 3. SCIT with Pru p 3, but not with R/A Pru p 3, suppressed anaphylaxis upon the challenge significantly. SCIT with Pru p 3 did not suppress Pru p 3-specific IgE and IgG1 production, but enhanced IgG2a production. In contrast, SCIT with R/A Pru p 3 suppressed IgE and IgG1 production, but enhanced IgG2a production only moderately. The therapeutic efficacy of SCIT with Pru p 3 was associated with induction of IL-10 and IFN-γ. Hypoallergenic folding variant of Pru p 3 is not likely an efficacious therapeutic component in SCIT of peach allergy. The lower efficacy of R/A Pru p 3 might be attributed to poor antigenicity and/or weak stability due to its unfolded conformation.

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Citations

Jun 18, 2019·Molecular Nutrition & Food Research·Stephanie EichhornGabriele Gadermaier
Jun 3, 2021·Foods·Cristobalina MayorgaFrancisca Gómez
Dec 28, 2019·Current Pediatric Reviews·Fernanda ChieraGiovanni Battista Pajno

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Methods Mentioned

BETA
Protein Assay
Assay
ELISA
Circular dichroism
gel filtration
flow cytometry

Software Mentioned

FlowJo®

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