Impact-induced muscle damage and urinary pterins in professional rugby: 7,8-dihydroneopterin oxidation by myoglobin

Scandinavian Journal of Medicine & Science in Sports
Angus LindsaySteven Gieseg

Abstract

Muscle damage caused through impacts in rugby union is known to increase oxidative stress and inflammation. Pterins have been used clinically as markers of oxidative stress, inflammation, and neurotransmitter synthesis. This study investigates the release of myoglobin from muscle tissue due to force-related impacts and how it is related to the subsequent oxidation of 7,8-dihydroneopterin to specific pterins. Effects of iron and myoglobin on 7,8-dihydroneopterin oxidation were examined in vitro via strong cation-exchange high-performance liquid chromatography (SCX-HPLC) analysis of neopterin, xanthopterin, and 7,8-dihydroxanthopterin. Urine samples were collected from 25 professional rugby players pre and post four games and analyzed for myoglobin by enzyme-linked immunosorbent assay, and 7,8-dihydroneopterin oxidation products by HPLC. Iron and myoglobin oxidized 7,8-dihydroneopterin to neopterin, xanthopterin, and 7,8-dihydroxanthopterin at concentrations at or above 10 μM and 50 μg/mL, respectively. All four games showed significant increases in myoglobin, neopterin, total neopterin, biopterin, and total biopterin, which correlated between each variable (P < 0.05). Myoglobin and iron facilitate 7,8-dihydroneopterin oxidation ...Continue Reading

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Oct 31, 2014·Journal of Sports Sciences·Angus LindsaySteven P Gieseg

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Citations

Apr 5, 2016·Oxidative Medicine and Cellular Longevity·Jelena PetrovićVesna Pešić
Jun 14, 2016·Sports Medicine·Angus Lindsay, Joseph T Costello
Apr 21, 2016·Experimental Physiology·Angus LindsaySteven P Gieseg
Feb 9, 2017·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Angus LindsaySteven P Gieseg
Sep 6, 2019·Journal of Clinical Medicine·Angus LindsaySteven P Gieseg
Jun 29, 2018·Antioxidants·Steven P GiesegAngus Lindsay
Sep 11, 2019·Journal of Science and Medicine in Sport·Angus Lindsay, Steven P Gieseg

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