Impact of 5 fluorouracil chemotherapy on gut inflammation, functional parameters, and gut microbiota

Brain, Behavior, and Immunity
A T SougiannisE A Murphy

Abstract

Emerging evidence suggests that gut microbiota may influence the response to chemotherapy. We sought to characterize the effects of 5 fluorouracil (5FU) chemotherapy on colon inflammation and functional measures in colorectal cancer (CRC) and to further determine whether gut microbiota can influence this response. 50 C57BL/6 were randomized into four groups; Control + Vehicle (n = 10), Control + 5FU (n = 10), AOM/DSS + Vehicle (n = 15), and AOM/DSS + 5FU (n = 15). CRC was induced chemically by a single 10 mg/kg injection of azoxymethane (AOM) followed by two cycles (2% and 1%) of dextran sodium sulfate (DSS). Mice were then treated with 3 cycles of vehicle or 5FU (cycle 1: 40 mg/kg, cycle 2 + 3: 20 mg/kg). Functional tests (grip strength and run-to-fatigue) were performed prior to 5FU treatment (baseline) and at the completion of the second cycle of 5FU. Following the third 5FU cycle, mice were euthanized and the colon was evaluated for expression of inflammatory genes using RT-qPCR and stool samples were profiled using 16S rRNA sequencing. A second experiment used fecal microbiota transplantation from 5FU treated mice to control mice (n = 10-15/group) to determine whether 5FU associated changes in the microbiota could influenc...Continue Reading

Citations

Dec 12, 2019·Scandinavian Journal of Gastroenterology·Lauren C ChartierSuzanne Mashtoub
Sep 29, 2020·Frontiers in Physiology·Brandon N VanderVeenJames A Carson
Aug 28, 2020·Journal of Applied Physiology·Alexander T SougiannisE Angela Murphy
Dec 29, 2020·Frontiers in Physiology·Brandon N VanderVeenE Angela Murphy
Jan 30, 2021·BMC Pharmacology & Toxicology·Alexander T SougiannisE Angela Murphy
Jan 21, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alexander T SougiannisE Angela Murphy
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Oumaima ZidiSoumaya Kouidhi
Jul 25, 2021·Cancers·Dean G CampeljEmma Rybalka
Nov 12, 2021·The British Journal of Radiology·Tianhe YeXiangwen Xia
Jan 6, 2022·Integrative Cancer Therapies·Sierra J McDonaldE Angela Murphy
Jan 13, 2022·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alexander T SougiannisE Angela Murphy

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