Abstract
The formation of robust resting cysts enables Acanthamoeba to resist harsh environmental conditions. This study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. Moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cross-tolerance toward other stressors. Several physical and chemical stressors (NaCl, H2O2, benzalkonium chloride, 55°C, heating until boiling, ethanol, dishwashing detergent, and sodium hypochlorite) frequently used in domestic and industrial food-related environments were tested against (i) Acanthamoeba castellanii cysts, (ii) single strains of bacterial monocultures, (iii) intracystic bacteria, and (iv) bacteria after intracystic passage (cyst-primed bacteria). Only heating until boiling and hypochlorite treatment were cysticidal. After boiling, no viable trophozoites could be recovered from the cysts, and hypochlorite treatment caused a 1.34- to 4.72-log10 cells/ml reduction in cyst viability. All treatments were effective in reducing ...Continue Reading
References
Jan 1, 1976·Annual Review of Microbiology·R A Weisman
Oct 1, 1991·Journal of General Microbiology·B M MackeyD A Seymour
Dec 1, 1988·Applied and Environmental Microbiology·C H KingK G Porter
Jan 1, 1997·Advances in Applied Microbiology·D G Ahearn, M M Gabriel
Nov 20, 1998·The Journal of Infection·W KhunkittiA D Russell
Jul 7, 2000·The Journal of Antimicrobial Chemotherapy·N A TurnerD Lloyd
Feb 27, 2001·The British Journal of Ophthalmology·S Kilvington, C Anger
Jun 16, 2001·Antimicrobial Agents and Chemotherapy·R Hughes, S Kilvington
Apr 27, 2002·Applied and Environmental Microbiology·Markus LinderEwert Linder
Jul 9, 2002·The Journal of Parasitology·A AksozekH Alizadeh
Jul 5, 2003·Journal of Clinical Microbiology·Tara K BeattieAnthony M Grimason
Sep 25, 2003·Antimicrobial Agents and Chemotherapy·Reanne HughesSimon Kilvington
Nov 1, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Gilbert Greub, Didier Raoult
Sep 17, 2004·Scandinavian Journal of Infectious Diseases·Michael V StoreyThor-Axel Stenström
Feb 16, 2005·Annual Review of Physiology·Dietmar Kültz
Apr 9, 2005·The Journal of Eukaryotic Microbiology·Bibiana Chávez-MunguíaAdolfo Martínez-Palomo
Sep 8, 2006·Applied and Environmental Microbiology·Toïdi AdékambiMichel Drancourt
Oct 17, 2008·Journal of Clinical Microbiology·Rama SriramGovinda S Visvesvara
Feb 4, 2009·Journal of Applied Microbiology·M Critchley, R Bentham
Sep 12, 2009·FEMS Microbiology Reviews·Vincent ThomasJean-Yves Maillard
Jun 4, 2010·Journal of Clinical Microbiology·Céline CoulonVincent Thomas
Jul 16, 2010·Food Microbiology·Ihab HabibLieven De Zutter
Jan 11, 2011·Applied and Environmental Microbiology·Nadia GruzdevShlomo Sela
Feb 23, 2012·The Journal of Eukaryotic Microbiology·Celine CoulonVincent Thomas
Mar 14, 2012·Journal of Food Protection·M J M VaerewijckK Houf
Apr 26, 2012·The Journal of Antimicrobial Chemotherapy·Ezra LinleyJean-Yves Maillard
Aug 13, 2013·International Journal of Hygiene and Environmental Health·Mathieu DupuyYann Héchard
Aug 13, 2013·Applied and Environmental Microbiology·E LambrechtK Houf
Jun 14, 2015·Applied and Environmental Microbiology·Ellen LambrechtKurt Houf
Jan 25, 2016·International Journal of Food Microbiology·Catherine M BurgessOdile Tresse
Citations
Apr 26, 2019·Journal of Virology·Iara BorgesJônatas Santos Abrahão
Feb 6, 2020·Journal of Applied Microbiology·A A Gabriel, D C Panaligan
Nov 7, 2019·Virology Journal·Graziele OliveiraJônatas Abrahão
Feb 23, 2020·Irish Veterinary Journal·Elvira RamovicDeirdre M Prendergast