Impact of Aging on Calcium Signaling and Membrane Potential in Endothelium of Resistance Arteries: A Role for Mitochondria

The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Erik J Behringer, Steven S Segal

Abstract

Impaired blood flow to peripheral tissues during advanced age is associated with endothelial dysfunction and diminished bioavailability of nitric oxide (NO). However, it is unknown whether aging impacts coupling between intracellular calcium ([Ca2+]i) signaling and small- and intermediate K+ channel (SKCa/IKCa) activity during endothelium-derived hyperpolarization (EDH), a signaling pathway integral to dilation of the resistance vasculature. To address the potential impact of aging on EDH, Fura-2 photometry and intracellular recording were applied to evaluate [Ca2+]i and membrane potential of intact endothelial tubes (width, 60 µm; length, 1-3 mm) freshly isolated from superior epigastric arteries of young (4-6 mo) and old (24-26 mo) male C57BL/6 mice. In response to acetylcholine, intracellular release of Ca2+ from the endoplasmic reticulum (ER) was enhanced with aging. Further, treatment with the mitochondrial uncoupler FCCP evoked a significant increase of [Ca2+]i with membrane hyperpolarization in an SKCa/IKCa-dependent manner in the endothelium of old but not young mice. We conclude that the ability of resistance artery endothelium to release Ca2+ from intracellular stores (ie, ER and mitochondria) and hyperpolarize Vm via...Continue Reading

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Citations

Mar 28, 2018·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Gwenael LayecRussell S Richardson
Nov 10, 2018·Journal of Applied Physiology·Matthew J Socha, Steven S Segal
Jan 26, 2020·Antioxidants·Magdalena RudzińskaAndrey A Zamyatnin
Jan 30, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Erika M Boerman, Steven S Segal
Jan 1, 2020·International Journal of Molecular Sciences·Tomasz BoczekLudmila Zylinska
Mar 22, 2019·International Journal of Molecular Sciences·Erik J Behringer, Md A Hakim
Nov 25, 2019·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Md A HakimErik J Behringer
May 27, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yong-Jia SongXue-Jun Cui
Oct 8, 2020·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Suraj KrishnanRajesh Mohandas
Dec 9, 2020·International Journal of Molecular Sciences·Chi-Hsiao YehTing-Fen Tsai

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