Impact of Aging on the Phenotype of Invariant Natural Killer T Cells in Mouse Thymus.

Frontiers in Immunology
Georgia PapadogianniHristo Georgiev

Abstract

Invariant natural killer T (iNKT) cells represent a subclass of T cells possessing a restricted repertoire of T cell receptors enabling them to recognize lipid derived ligands. iNKT cells are continuously generated in thymus and differentiate into three main subpopulations: iNKT1, iNKT2, and iNKT17 cells. We investigated the transcriptomes of these subsets comparing cells isolated from young adult (6-10 weeks old) and aged BALB/c mice (25-30 weeks of age) in order to identify genes subject to an age-related regulation of expression. These time points were selected to take into consideration the consequences of thymic involution that radically alter the existing micro-milieu. Significant differences were detected in the expression of histone genes affecting all iNKT subsets. Also the proliferative capacity of iNKT cells decreased substantially upon aging. Several genes were identified as possible candidates causing significant age-dependent changes in iNKT cell generation and/or function such as genes coding for granzyme A, ZO-1, EZH2, SOX4, IGF1 receptor, FLT4, and CD25. Moreover, we provide evidence that IL2 differentially affects homeostasis of iNKT subsets with iNKT17 cells engaging a unique mechanism to respond to IL2 by in...Continue Reading

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Citations

Oct 24, 2021·International Immunology·Nanami MinoHiroshi Takayanagi

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Datasets Mentioned

BETA
GSE147666

Methods Mentioned

BETA
FCS
FACS
PMA
RNA-Seq
PCR
DNA Assay
Assay
flow cytometry
single cell sequencing
flow

Software Mentioned

STAR
FastQC
Cytek Aurora
featureCounts
Gzma
GraphPad Prism
Trim Galore !
Galaxy
Galaxy Tool
DESeq2 Galaxy Tool

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