PMID: 2502393Jul 1, 1989Paper

Impact of altered protein structures on the intracellular traffic of a mutated vasopressin precursor from Brattleboro rats

European Journal of Biochemistry
H SchmaleD Richter

Abstract

The rat vasopressin precursor, synthesized in the reticulocyte lysate system under the direction of in vitro transcribed mRNA, is processed and correctly delivered to the lumen of added microsomal vesicles. Translation of mRNA for the mutant (Brattleboro) vasopressin precursor which lacks a translational stop codon as a consequence of a frame-shift mutation, gives rise to a mutated protein (B-mutant precursor) with a C-terminal poly(lysine) sequence encoded by the poly(A) tail. Upon addition of microsomal membranes, the mutated precursor has access to the lumen of the vesicles as indicated by removal of the signal peptide; however, the C-terminal part with the poly(lysine) tail remains outside the vesicles as shown by its sensitivity to proteinase K. When a modified RNA, including a stop codon located similarly to that found in the cDNA encoding the normal precursor, is translated in the presence of microsomal membranes, the resulting product (S-mutant precursor) is refractory to proteolysis by exogenously added proteinase K. Analysis of the microsomal membranes indicates, however, that the C-terminus of the S-mutant precursor is still anchored within membranes. For studying the intracellular transport of the mutated precursor ...Continue Reading

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Citations

Apr 29, 1993·Regulatory Peptides·E Mohr, D Richter
Oct 1, 1995·Mutation Research·D A EvansF W van Leeuwen
May 1, 1995·Trends in Cardiovascular Medicine·M Paul, W M Franz
Jun 28, 2003·Brain Research. Molecular Brain Research·Evita Mohr, Dietmar Richter
Dec 22, 2000·Neurobiology of Aging·F W van LeeuwenE M Hol
Apr 24, 1999·Progress in Neurobiology·E Mohr
Jun 10, 2000·Journal of Neuroendocrinology·D A EvansJ P Burbach
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·D A EvansF W van Leeuwen
Jan 1, 1992·The European Journal of Neuroscience·Evita Mohr, Dietmar Richter
May 5, 2000·Experimental Physiology·F D Grant
Jul 21, 2001·Seminars in Cell & Developmental Biology·P H Taghert
Oct 1, 1993·Hypertension·F D GrantJ A Majzoub
May 3, 2016·Biomolecules & Therapeutics·Ki Chan KimChan Young Shin
Jul 27, 2000·Annals of the New York Academy of Sciences·F W van LeeuwenE M Hol
Aug 6, 1999·La Revue de médecine interne·C Ajzenberg

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