Impact of Arsenite on the Bacterial Community Structure and Diversity in Soil

Microbes and Environments
Dian-Tao DongSeigo Amachi

Abstract

The impact of arsenite (As[III]) on the bacterial community structure and diversity in soil was determined by incubating soil slurries with 50, 500, and 5,000 μM As(III). As(III) was oxidized to arsenate (As[V]), and the microbial contribution to As(III) oxidation was 70-100%. PCR-denaturing gradient gel electrophoresis revealed that soil bacterial diversity decreased in the presence of As(III). Bacteria closely related to the family Bacillaceae were predominant in slurry spiked with 5,000 μM As(III). The population size of culturable As(III)-resistant bacteria was 37-fold higher in this slurry than in unspiked slurry (p < 0.01), indicating that high levels of As(III) stimulate the emergence of As(III)-resistant bacteria. As(III)-resistant bacteria isolated from slurry spiked with 5,000 μM As(III) were mainly affiliated with the genus Bacillus; however, no strains showed As(III)-oxidizing capacity. An As(III)-oxidizing bacterial community analysis based on As(III) oxidase gene (aioA) sequences demonstrated that diversity was the lowest in slurry spiked with 5,000 μM As(III). The deduced AioA sequences affiliated with Alphaproteobacteria accounted for 91-93% of all sequences in this slurry, among which those closely related to B...Continue Reading

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Datasets Mentioned

BETA
LC012044
LC027609
LC051121
LC051138

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

R
BLASTP
MEGA
Clustal W
one
Clustal X
Basic Local Alignment Search Tool ( BLAST )
Quantity

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