Abstract
Malondialdehyde (MDA), glyoxal (GO), and methylglyoxal (MGO) levels increase in atherosclerosis and diabetes patients. Recent reports demonstrate that GO and MGO cause vascular endothelial barrier dysfunction whereas no evidence is available for MDA. To compare the effects of MDA, GO, or MGO on endothelial permeability, we used human EA.hy926 endothelial cells as a standard model. To study cortical cytoplasm motility and cytoskeletal organization in endothelial cells, we utilized time-lapse microscopy and fluorescent microscopy. To compare dicarbonyl-modified protein band profiles in these cells, we applied Western blotting with antibodies against MDA- or MGO-labelled proteins. MDA (150-250 μM) irreversibly suppressed the endothelial cell barrier, reduced lamellipodial activity, and prevented intercellular contact formation. The motile deficiency of MDA-challenged cells was accompanied by alterations in microtubule and microfilament organization. These detrimental effects were not observed after GO or MGO (250 μM) administration regardless of confirmed modification of cellular proteins by MGO. Our comparative study demonstrates that MDA is more damaging to the endothelial barrier than GO or MGO. Considering that MDA endogenous ...Continue Reading
References
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 15, 1970·Nature·U K Laemmli
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J EdgellJ B Graham
Jul 1, 1996·Analytical Biochemistry·F W ChaplenD C Cameron
Feb 15, 1997·The Biochemical Journal·J R RequenaS R Thorpe
Feb 17, 1998·Neuroscience Letters·A R HipkissN J Abbot
Apr 17, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·E G VermeulenV W van Hinsbergh
Jul 22, 2004·Experimental Cell Research·Dmitry S KudryashovVladimir P Shirinsky
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Oct 10, 2006·European Journal of Pharmacology·William C S ChoKevin K M Yue
Mar 6, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jens Lykkesfeldt
Jul 9, 2009·Molecular and Cellular Biochemistry·Sean M SlimanNarasimham L Parinandi
Jan 18, 2011·Chemico-biological Interactions·Reinald Pamplona
Jan 6, 2012·Doklady. Biochemistry and Biophysics·V Z LankinS N Pokrovsky
Jan 25, 2012·Diabetologia·T FlemingP P Nawroth
May 9, 2012·Advances in Urology·Jessica N LangeRoss P Holmes
Dec 25, 2012·Journal of Lipid Research·Hamdy F MoselhySusanne P Boyle
Feb 27, 2013·Journal of Proteomics·Neven ZarkovicKamelija Zarkovic
Mar 16, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Jean L J M Scheijen, Casper G Schalkwijk
May 25, 2013·Journal of Proteomics·Andreas Simm
Jul 6, 2014·Molecular and Cellular Biochemistry·Vadim LankinMargus Viigimaa
Jul 8, 2014·Oxidative Medicine and Cellular Longevity·Antonio AyalaSandro Argüelles
Jul 18, 2014·PloS One·Andrea E TóthMária A Deli
Jul 30, 2014·Molecular and Cellular Biochemistry·Elena M KumskovaVadim Z Lankin
Dec 3, 2014·Archives of Medical Research·Andrea E TóthMária A Deli
May 21, 2015·Journal of Diabetes·Vadim Z LankinMargus Viigimaa