Impact of benznidazole treatment on the functional response of Trypanosoma cruzi antigen-specific CD4+CD8+ T cells in chronic Chagas disease patients

PLoS Neglected Tropical Diseases
Elena Pérez-AntónManuel Carlos López

Abstract

Chagas disease is caused by Trypanosoma cruzi. The persistence of the parasite is associated with the disease chronicity and the impairment of the cellular immune response. It has been reported that the CD4+CD8+ T cell population expands in chronic Chagas disease patients. Few studies have focused on this subset of cells, and very little is known about the impact of antiparasitic treatment on this population. Thirty-eight chronic Chagas disease patients (20 asymptomatic and 18 symptomatic) and twelve healthy controls were enrolled in this study. Peripheral blood mononuclear cells were stimulated with soluble T. cruzi antigens to analyze the production of cytokines and cytotoxic molecules by CD4+CD8+ T cells before and after benznidazole treatment. Additionally, expression and co-expression of five inhibitory receptors in these patients after treatment were studied using a multiparameter flow cytometry technique. The frequency of CD4+CD8+ T cells was higher in chronic Chagas disease patients compared with healthy donors. Furthermore, a higher ratio of CD4+CD8low/CD4+CD8high subpopulations was observed in chronic Chagas disease patients than in healthy donors. Additionally, CD4+CD8+ T cells from these patients expressed and co-ex...Continue Reading

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Citations

Jul 30, 2020·Revista Do Instituto De Medicina Tropical De São Paulo·Virgínia Paula FradeCláudia Di Lorenzo Oliveira
Nov 2, 2018·Current Medicinal Chemistry·Adriana EguiManuel Carlos López
Sep 13, 2019·Acta Tropica·Lizette E RiosNisha J Garg
Dec 21, 2020·Immunobiology·Ana K A SoaresVirginia M B Lorena
Jun 2, 2021·PLoS Neglected Tropical Diseases·María Ailén NataleSusana Adriana Laucella
Oct 30, 2021·Frontiers in Cellular and Infection Microbiology·Jose MateusAdriana Cuéllar

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Methods Mentioned

BETA
PCR
protein assay
flow cytometry

Software Mentioned

SPICE
GraphPad Prism
FlowJo
GraphPad

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