Impact of Bioinspired Nanotopography on the Antibacterial and Antibiofilm Efficacy of Chitosan

Biomacromolecules
Abinash TripathyProsenjit Sen

Abstract

Chitosan derived from chitin is one of the most abundant naturally occurring biocompatible polymers obtained from fungi and arthropods. In this work, we report the enhancement in the bactericidal efficacy of CHI in the presence of a sharp nanotopography. High-aspect ratio nanostructured surface (NSS) was fabricated using a single-step deep reactive ion etching technique (DRIE). Post fabrication, CHI coating was carried out using a layer-by-layer (LBL) dip coating process on the flat and nanostructured surfaces. Antibacterial efficacy of the flat silicon surface coated with CHI (Si_CHI) and NSS coated with CHI (NSS_CHI) was tested against both Gram-negative (G-ve) bacteria E. coli and Gram-positive (G+ve) bacteria S. aureus. NSS_CHI exhibited superior antibacterial property against G-ve and G+ve microbes as compared with Si_CHI and NSS substrates. Scanning electron microscopy (SEM) and fluorescence microscopy were used to study the morphology and viability of the bacteria on all the surfaces. Also, biofilm quantification was carried out on all the engineered surfaces for both E. coli and S. aureus using crystal violet (CV) staining. NSS_CHI was found to have the minimum biofilm formation on its surface exhibiting its superior an...Continue Reading

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Citations

Dec 18, 2019·Journal of Endourology·Diamela M RoccaM Cecilia Becerra
Aug 25, 2020·Industrial & Engineering Chemistry Research·Athanasios MilionisDimos Poulikakos
Jun 11, 2018·ACS Biomaterials Science & Engineering·Abinash TripathyProsenjit Sen
Aug 22, 2021·Macromolecular Rapid Communications·Jielin MaHongwei Duan
Dec 15, 2021·ACS Applied Materials & Interfaces·Rujian JiangLuquan Ren

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