Impact of bone marrow hematopoiesis failure on T-cell generation during pathogenic simian immunodeficiency virus infection in macaques.

Blood
H ThiebotRoger Le Grand

Abstract

Experimental infection of macaques with pathogenic strains of simian immunodeficiency virus (SIV) represents one of the most relevant animal models for studying HIV pathogenesis. In this study, we demonstrated a significant decrease in the generation of CD4+ T cells from bone marrow (BM) CD34+ progenitors in macaques infected with SIVmac251. This decrease appears to result from changes in the clonogenic potential of BM progenitors of both the myeloid and lymphoid lineages. We also demonstrated a significant decrease in the numbers of the most immature long-term culture-initiating cells (LTC-ICs). Hematopoietic failure occurred as early as primary infection, in the absence of CD34+ BM cell infection and was not related to plasma viral load. No major change was observed in the phenotype of BM CD34+ cells from infected macaques, including apoptosis markers such as annexin V staining and BcL-2 expression, but a significantly higher that normal proportion of cells were in the G0/G1 phase. This is the first demonstration that failure of BM hematopoiesis results in impaired T-cell production, which may contribute to the disruption of T-lymphocyte homeostasis characteristic of pathogenic lentiviral infections in primates.

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Citations

May 9, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Raffaele Badolato
Apr 26, 2012·Applied Immunohistochemistry & Molecular Morphology : AIMM·Brijal DaveParul Bhargava
Sep 22, 2005·Immunology and Cell Biology·Michaela Müller-Trutwin, Anne Hosmalin
Mar 26, 2009·Retrovirology·Carole ElbimJérome Estaquier
Mar 19, 2008·PloS One·Anna Le TortorecNathalie Dejucq-Rainsford
May 8, 2010·Zoological Research·Hou-Jun XiaYong-Tang Zheng
Mar 25, 2014·International Reviews of Immunology·Xin ShiPing Zhang
Jan 26, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Patrick AutissierKenneth C Williams
Oct 2, 2015·Health Physics·Ann M FareseThomas J MacVittie
Aug 19, 2006·Journal of Leukocyte Biology·Anne Hosmalin, Pierre Lebon
Dec 24, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arielle Glatman ZaretskyChristopher A Hunter
Mar 11, 2020·Frontiers in Cellular and Infection Microbiology·Tetsuo Tsukamoto

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