Impact of cigarette-smoking on sperm DNA methylation and its effect on sperm parameters

Andrologia
Y AlkhaledM E Hammadeh

Abstract

DNA methylation is an epigenetic modification of the genome. The purpose of this study was to determine the influence of cigarette-smoking on sperm DNA methylation from a genomewide survey of sperm samples and to ascertain its effect on sperm parameters. Twenty-eight sperm DNA samples (from 14 fertile smokers as a case study and 14 proven fertile nonsmokers as controls) were subjected to Infinium 450K BeadChip arrays to identify the changes in the DNA methylation level between the two groups. Then, deep bisulphite sequencing was used to validate five CpGs on 78 samples. The results from the Infinium 450K found that only 11 CpGs showed a significant difference in DNA methylation between the case and the control groups. Five CpGs of the eleven (cg00648582, cg0932376, cg19169023, cg23841288 and cg27391564) underwent deep bisulphite sequencing where cg00648582, related to the PGAM5 gene, and the cg23841288 CpGs, related to the PTPRN2 gene amplicons, showed a significant increase in their DNA methylation level in more than one CpG in the case group. In contrast, a significant decrease was found at cg19169023 and at its various neighbouring CpGs in the TYRO3 gene-related amplicons. Furthermore, this study demonstrated a significant c...Continue Reading

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Citations

Dec 7, 2018·Epigenetics : Official Journal of the DNA Methylation Society·Susan K MurphyScott H Kollins
Dec 15, 2019·Birth Defects Research·Sonsoles Navarro-Rubio, Francisco Güell
Jan 5, 2020·Andrology·Chelsea MarchoJ Richard Pilsner
Aug 14, 2020·Human Reproduction Update·Fredrika ÅseniusSarah J Marzi
Sep 27, 2019·Archives of Toxicology·Gagandeep KaurSanjay Batra
Feb 21, 2019·Clinical Epigenetics·Razia ZakaryaBrian G Oliver
Mar 5, 2021·Scientific Reports·Yung-Che ChenUNKNOWN Taiwan Clinical Trial Consortium of Respiratory Disease (TCORE) group
Sep 1, 2021·Journal of Applied Toxicology : JAT·Huage Liu, Hao Chen
Dec 3, 2021·Environmental Science and Pollution Research International·Mohammed M Laqqan, Maged M Yassin

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