Impact of cleaning monitoring combined with channel purge storage on elimination of Escherichia coli and environmental bacteria from duodenoscopes

Gastrointestinal Endoscopy
Harminder SinghMichelle J Alfa

Abstract

We aimed to determine whether monitoring of duodenoscope cleaning by rapid adenosine triphosphate (ATP) combined with channel-purge storage could eliminate high-concern microorganisms. In a simulated-use study, suction channels, as well as lever recesses, from 2 duodenoscopes models and the unsealed elevator guidewire (EGW) channel from 1 of these 2 duodenoscopes (the other model has a sealed EGW) were perfused with ATS2015 containing approximately 8 Log10 colony-forming units (CFU)/mL of both Enterococcus faecalis and Escherichia coli. Pump-assisted cleaning was monitored by rapid ATP testing. Duodenoscopes exceeding 200 relative light units (RLUs) were recleaned. Clean duodenoscopes were processed through an automated endoscope reprocessor and then stored in a channel-purge storage cabinet for 1 to 3 days. Cultures of EGW channel and instrument channel combined with the lever recess (IC-LR) were taken after storage. The impacts of extended cleaning and alcohol flush were evaluated. E coli was reliably eliminated in IC-LR and EGW channels of 119 duodenoscope tests (59 with sealed EGW and 60 with nonsealed EGW). However, actionable levels of E faecalis and environmental bacteria persisted. Neither alcohol flush nor extended cle...Continue Reading

Citations

Oct 23, 2018·Journal of Gastroenterology and Hepatology·Benedict M DevereauxRajvinder Singh
Sep 5, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Gheorghe G BalanAnca Trifan
Jun 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gheorghe G BalanCatalin Victor Sfarti
Oct 14, 2020·Biomedical Instrumentation & Technology·Camila Q M BrunaKazuko U Graziano
Oct 14, 2020·Biomedical Instrumentation & Technology·Jahan AziziMary Ann Drosnock
Sep 7, 2021·Gastrointestinal Endoscopy·Mark A Gromski, Stuart Sherman

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